用基因芯片技术分析不同剂量^(60)Coγ射线照射后淋巴细胞株的差异基因表达谱  

Detection on the differential gene expression profiling of lymphoblastoid cells induced by different dose levels of ^(60)Co γ-rays

作　　者：陆雪[1] 李玉文[1] 封江彬[1] 陈德清[1] 刘青杰[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《辐射研究与辐射工艺学报》2009年第4期244-247,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30870749;30570551)资助

摘　　要：用基因芯片来筛选不同剂量辐射诱导的差异表达基因,为快速生物剂量指标的筛选提供科学依据。用0.5、3和8Gy 60Coγ射线照射指数增长期的淋巴细胞永生化细胞株,24h后提取细胞总RNA、反转录成cDNA、标记后用基因芯片筛选各剂量点的差异表达基因;并用实时荧光PCR对某些基因表达水平进行定量分析。结果发现,0.5、3和8Gy剂量组分别有16、240和462个差异表达基因,其中在三个剂量组均上调或下调的基因分别为4个和3个。对TP53I3基因进行实时荧光PCR定量分析,结果与芯片结果一致。电离辐射诱导的TP53I3、GDF15、CDC43EP5、S100A4、IGJ和KLF2等基因的mRNA表达水平变化有可能与照射剂量有关。Differential gene expression profiting induced by different dose levels was detected with cDNA microarray, in order to search the possible radiation biodosimeters. Lymphoblastoid cells were irradiated with 0, 0.5, 3 or 8Gy 60Coγ-rays. Total RNA was extracted in 24h after irradiation, cDNA was synthesized and labeled with biotin, and then gene expression profiling was detected with cDNA microarray. Differential expressed gene detected with microarray was certified by real-time PCR. The experimental results show that 16, 240 and 462 differential expressed genes have been detected at 0.5, 3 and 8Gy, respectively. Four genes are up-regulated and three genes are down-regulated in all the three dose groups. The dose-dependent inducible expression of TP5313 gene has been further confirmed by real-time PCR. The expression changes of TP5313, GDF15, CDC42EP5, S100A4, IGJ and KLF2 genes after irradiation might play a role in radiation-induced bioeffects.

关 键 词：基因芯片 差异表达基因 淋巴细胞 60Coγ射线 

分 类 号：R811.5[医药卫生—放射医学] Q691.5[医药卫生—临床医学]