慢性应激对小鼠学习记忆及免疫细胞的影响  

Effect of Chronic Psychological Stress on Learning and Memory in Mice and the Impact of Immune Cells

作　　者：马颖[1] 卢莉[2] 薛朝霞[2] 袁洁[2] 

机构地区：[1]山西医科大学第一医院神经外科,山西太原030001 [2]山西医科大学医学心理学教研室,山西太原030001

出　　处：《中国临床心理学杂志》2009年第4期394-395,399,共3页Chinese Journal of Clinical Psychology

基　　金：山西省自然科学基金资助项目(051089)

摘　　要：目的:观察慢性应激对小鼠学习记忆的影响以及脾组织中T细胞亚群CD4、CD8水平的变化。方法:采用束缚制动的方法建立慢性应激模型,应激结束后采用Morris水迷宫实验观察应激状态下小鼠的学习记忆能力;处死,采用流式细胞仪检测小鼠脾组织中CD4+T细胞、CD8+T细胞的数量以及CD4/CD8比值。结果:应激组小鼠在Morris水迷宫中逃避潜伏期较对照组显著延长(P<0.01),第一象限的游泳时间比例显著小于对照组(P<0.01),小鼠脾淋巴细胞悬液中CD4+T细胞明显低于对照组(P<0.05),而CD8+T细胞高于对照组(P<0.05)及CD4/CD8比值低于对照组(P<0.05)。结论:慢性应激可以导致机体学习记忆能力减退,抑制细胞免疫功能,容易导致机体产生疾病。Objective： To observe the effect of chronic psychological stress on change of function of learning and memory, and CD4, and CD8 in mice. Methods： 50 BALB/c mice were randomly divided into control group and immobilization stress group （n=25 in each group）. The mice mode of stress was made by restraint for 6 hours per day. Suspend tail test was performed on change of behaviors in mice. RIA was employed to measure levels of neuropeptide Y in the brain. Results： Compared with the control group, the mean escape latency of the mice in the chronic restraint groups was longer than that of the control mice （P〈0.01）, the average percentages of the swimming time in the platform quadrant in the restraint group were less than those in the control group （P〈0.01）. Chronic stress significantly decreased levels of CIM （P〈0.05）, and CD/CD8 （P〈0.05）, while CD8 increased. Conclusion： Chronic psychological stress can lead to learning and memory dimish, and inhibit the cellular immune function, thus the body easily lead to a disease.

关 键 词：慢性应激 学习记忆 免疫细胞 

分 类 号：R395.1[哲学宗教—心理学]