生物蛋白胶对家兔大腿伤口愈合中Smad3表达影响的研究  

The investigation of the effect of bioprotein mastic on the expression of Smad3 in rabbit thigh wound healing

作　　者：李欣[1] 王鑫[1] 陈戈[1] 马志坚[1] 廖鹏[1] 赵航[1] 杨洪昌[1] 汪颖[1] 杨华刚[1] 李涛[1] 季建华[1] 

机构地区：[1]云南省第二人民医院创伤救治中心,云南昆明650021

出　　处：《云南医药》2009年第4期404-406,共3页Medicine and Pharmacy of Yunnan

摘　　要：目的探讨生物蛋白胶对兔大腿伤口愈合中Smad3表达的影响。方法将50只实验家兔分为正常创面组\使用生物蛋白胶组。以家兔右大腿作深度达肌层的创面为模型,各组分别给予简单清创、缝合\清创、喷洒生物蛋白胶、缝合的处理措施后,应用免疫组化、图像分析、Westernblot等技术,观察各组家兔创面肉芽组织中Smad3表达水平的变化。结果Westernblot检测结果显示,喷洒生物蛋白胶创面组Smad3的表达水平明显低于正常创面组(P<0.01)。结论生物蛋白胶能促进家兔大腿伤口愈合,下调Smad3的表达水平是其作用机制之一。Objective To study the effect of bioprotein mastic on the expression of Smad3 in rabbit thigh wound healing. Methods 50 experimental rabbits were divided into common wound group and bioprotein mastic treated group. A wound that deep to muscle was made in right thigh of each rabbit. Common wound group treated with normal debride and suture, and the experimental group treated with debride,insufflation bioprotein mastic on surface of wound and suture. The changes of expression level of Smad3 in rabbits wound granulation tissue were observed by immunity class, image analysis, and Western blot technique. Results The result of Western blot technique showed that the expression level of Smad3 in bioprotein mastic treated group was obviously lower than in common wound group （P〈0.01） Conclusion Bioprotein mastic can promote the healing of wound on rabbit right thigh. The Smad3 expression level lowering is one of the bioprotein mastic＇s function.

关 键 词：生物蛋白胶 SMAD3 创口愈合 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]