补肾通脉方对伴胰岛素抵抗的多囊卵巢综合征大鼠胰岛素抵抗及排卵障碍的影响  

Effect of Bushen Tongmai Recipe on Insulin Resistance and Ovulation Dysfunction in PCOS Rats Accompanied with Insulin Resistance

作　　者：李琼[1] 黄冬梅[1] 陆付耳[1] 谢阳[1] 徐丽君[1] 邹欣[1] 龚菂[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030

出　　处：《中国中西医结合杂志》2009年第8期733-736,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题(No.30600838)

摘　　要：目的建立由硫酸普拉睾酮钠联合高脂饮食诱导的伴胰岛素抵抗(insulin resistance,IR)的多囊卵巢综合征(polycystic ovarian syndrome,PCOS)的大鼠模型,并观察补肾通脉方对模型大鼠IR和排卵障碍的影响。方法23日龄雌性SD大鼠皮下注射硫酸普拉睾酮钠每天9mg/100g,共20天,联合高脂饮食喂养80天,建立伴IR的PCOS大鼠模型,成模大鼠随机分为模型组和治疗组。治疗组大鼠以补肾通脉方进行干预。另设15只同龄大鼠为正常组。HE染色观察大鼠卵巢的组织学改变及排卵情况;葡萄糖氧化酶法测定空腹血糖(FBG);放射免疫法测定空腹血清胰岛素(FINS)、睾酮(T)、雌二醇(E2)、孕酮(P)、促卵泡刺激素(FSH)和黄体生成素(LH)的含量。结果与正常组比较,模型组大鼠平均排卵数、排卵率、胰岛素敏感指数(ISI)及FSH均明显降低(均P<0.01),FBG、FINS、T、E2和LH浓度均显著升高(P<0.05,P<0.01);与模型组比较,治疗组大鼠平均排卵数及排卵率均明显增加(均P<0.01),ISI、FSH显著升高(P<0.01),FINS、T和E2浓度均显著降低(P<0.01)。结论硫酸普拉睾酮钠联合高脂饮食能成功建立伴IR的PCOS大鼠模型,补肾通脉方对模型大鼠的IR有改善作用,并可促进其排卵。Objective To establish a rat model of polycystic ovarian syndrome accompanied with insulin resistance （PCOS-IR）, and to observe the effects of Bushen Tongmai Recipe （BSTMR） on insulin resistance and ovulation dysfunction in the model. Methods Sodium prasterone sulfate at the daily dose of 9 mg/100 g was subcutaneously injected to female SD rats, 23-day old, for 20 days, and fed with high-fat forage for 80 days to establish the rat model of PCOS-IR. The model rats were randomized into the model group and the treated group administered with BSTMR. Besides, a group consisted of 15 healthy rats was set up as a normal control. Ovarian histological changes and ovulation condition in rats were examined with hematoxylin and eosin （HE） staining; fasting blood glucose （FBG） was determined by glucose oxidase method; serum levels of fasting insulin （ FINS）, serum testosterone （T）, estradiol （ E2 ）, progesterone （ P）, follicle stimulating hormone （FSH） and luteotrophic hormone （LH） were detected by radioimmunoassay （RIA）. Results Compared with the normal group, in the model group, the mean number of corpus luteum, ovulation rate, FSH level and insulin sensitive index （ISI） were lower significantly （P〈0.01）, and levels of FBG, FINS, T, E2 and LH were higher （P〈0.05, P 〈0.01 ）. Compared with the model group, in the treated group, the mean number of corpus luteum, ovulation rate, levels of FSH and ISI were higher, and levels of FINS, T and E2 were lower （all P 〈 0.01 ）. Conclugion Rat model of PCOS-IR could be established by sodium prasterone sulfate and high-fat forage, and the insulin resistance and ovulation dysfunction in the model rats could be improved by BSTMR.

关 键 词：多囊卵巢综合征 胰岛素抵抗 排卵障碍 补肾通脉方 

分 类 号：R271.9[医药卫生—中西医结合]