组织工程骨神经化构建的组织学研究  

Histology research about nervalization of tissue engineered bones

作　　者：刘勇[1] 裴国献[2] 江汕[2] 姚旺祥[2] 任高宏[2] 孙新君[1] 

机构地区：[1]解放军第89医院全军创伤骨科研究所,山东潍坊261021 [2]南方医科大学南方医院创伤骨科,广州510515

出　　处：《中国矫形外科杂志》2009年第16期1246-1249,共4页Orthopedic Journal of China

基　　金：国家高技术研究发展计划(863计划)重大专项课题(编号:2003AA205001);国家自然科学基金资助项目(编号:30600643)

摘　　要：[目的]通过观察不同神经束植入组织工程骨后组织学的变化,探讨组织工程骨神经化构建的效果。[方法]将新西兰大白兔的骨髓基质干细胞(bone marrow stromal cells,BMSCs)进行诱导分化为成骨细胞,与β-磷酸三钙(β-tricalcium phosphate,β-TCP)复合制作组织工程骨,分别将不同类型神经束植入组织工程骨,进行以下分组:A组,组织工程骨组;B组,感觉神经束植入组;C组,运动神经束植入组;D组,血管束(含有自主神经)植入组;E组,感觉、运动神经束联合植入组。分别用于修复兔股骨1.5cm大段骨缺损;各组分别在12周进行组织学的Masson染色,降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的免疫组织化学检测,并进行统计学分析,以比较骨缺损修复情况。[结果]在观察期内,有感觉神经植入的组别和血管束植入的组别在组织学上具有明显骨化作用,而运动神经束植入却没有明显的促进作用。[结论]感觉神经束和血管束具有明显的促组织工程骨成骨作用,可以作为组织工程骨神经化构建的一种方法。[ Objective] To study the ossification effect by histology research on tissue engineered bone by transplanting nerval bundles of different types. [ Method] The Newzeland rabbit BMSCs （bone marrow stromal cells, BMSCs） were induced to osteoblast and compounded with β-TCP （β-tricalcium phosphate, β-TCP）to make tissue engineered bones, and different kinds of nerval bundles were transplanted into these tissue engineered bone. All Newzeland rabbits were divided into 5 groups according to different types of nerval bundles：Group A, tissue engineered bones without nerval bundles transplantation. Group B, tissue engineered bones transplanted with sensory nerval bundles. Group C, tissue engineered bones transplantation with motor nerval bundles. Group D, tissue engineered bones transplanted with vascular bundles （autonomic nerves contained）. Group E, tissue engineered bones transplantation with both sensory and motor nerval bundles. Then, 1.5 cm bone defect in femur of Newzeland rabbits were repaired. At 12 week,gross specimen observation, histology studies and immunohistochemistry were used to compare the results of bone repair in different groups. [ Result] During the observation period ,tissue engineered bones transplanted with sensory and vascular bundles effectively accelerated the ossification of the tissue engineered bones, while those transplanted with motor nerval bundles did not. [ Conclusion] Sensory nerval bundles and vascular bundles can effectively accelerate the ossification of tissue engineered bones. They can be used as a construction method for nervalization after tissue engineered bone.

关 键 词：组织工程骨 神经化 骨缺损 

分 类 号：R318[医药卫生—生物医学工程]