多价肺炎链球菌荚膜多糖结合物对小鼠免疫应答的影响  

Effect of Polyvalent Pneumococcal Capsular Polysaccharide Conjugates on Immune Response in Mice

作　　者：任克明[1] 陈晓航[1] 张轶[1] 刘爱萍[1] 陈美华[1] 王剑虹[1] 沈荣[1] 

机构地区：[1]兰州生物制品研究所第三研究室,兰州730046

出　　处：《中国生物制品学杂志》2009年第8期792-794,共3页Chinese Journal of Biologicals

摘　　要：目的探讨多价肺炎链球菌荚膜多糖结合物对小鼠免疫应答的影响。方法分别用单一载体(破伤风类毒素)和混合载体(破伤风类毒素和白喉类毒素)作为多糖结合物的载体蛋白,制备5～9价肺炎链球菌荚膜多糖结合物,免疫NIH小鼠,采用间接ELISA法测定免疫小鼠血清中抗荚膜多糖特异性IgG抗体水平。结果多价肺炎链球菌荚膜多糖结合物的免疫效果优于相应的单价结合物;随着结合物配制价数的增高,小鼠的免疫应答能力趋于下降;多价结合物中使用两种载体能缓解此现象,但载体含量过高时,依然会抑制免疫应答。结论多价肺炎链球菌荚膜多糖结合物免疫小鼠后,随着载体剂量的增高,会出现载体诱导的表位抑制现象,使小鼠的免疫应答反应减弱。两种载体蛋白同时使用能缓解此现象。Objective To investigate the effect of polyvalent pneumococcal capsular polysaccharide conjugates on immune response in mice. Methods Five- to nine-valent pneumococcal capsular polysaccharide conjugates were prepared using tetanus toxoid （TF） and TT ＋ diphtheria toxoid （DT） as carrier proteins separately, and used for immunization of NIH mice. The specific IgG levels against pneumococcal capsular polysaccharide in sera of immunized mice were determined by indirect ELISA. Results The immune effect of polyvalent pneumococcal capsular polysaccharide conjugates was superior to that of the corresponding monovalent conjugates. The immune response level of mice showed a decreasing tendency with the increasing number of valence of conjugates, which was relived by using TT ＋ DT as carriers. However, excessive carrier dosage still inhibited the immune response. Conclusion After immunization of mice with polyvalent pneumococcal capsular polysaccharide conjugates, epitope suppression induced by carrier proteins was observed with the increasing carrier dosage, resulting in decreased immune response level, which was relived by using two kinds of carrier proteins at the same time.

关 键 词：肺炎链球菌荚膜多糖 结合物 破伤风类毒素 白喉类毒素 免疫应答 表位抑制 

分 类 号：R392[医药卫生—免疫学]