辛伐他汀对体外培养系统性硬皮病患者的成纤维细胞增殖和胶原表达的影响  

Effects of Simvastatin on Cell Biological Activities and Procollagen Transcription of Dermal Fibroblasts in Systemic Scleroderma

作　　者：钱革[1] 李建国[1] 吴剑波[2] 周武庆[3] 王千秋[3] 禹卉千[1] 李振鲁[1] 

机构地区：[1]河南省人民医院皮肤科,河南郑州450003 [2]武汉大学中南医院皮肤科,湖北武汉430071 [3]中国医学科学院皮肤病研究所,江苏南京210042

出　　处：《中国皮肤性病学杂志》2009年第8期478-480,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨辛伐他汀对系统性硬皮病患者皮肤成纤维细胞增殖、胶原分泌和Ⅰ型前胶原mRNA表达的影响。方法原代培养系统性硬皮病患者皮肤成纤维细胞,检测不同浓度的辛伐他汀作用于体外培养的成纤维细胞48h后细胞的存活力;观察不同浓度的辛伐他汀对细胞胶原分泌和Ⅰ型前胶原mRNA合成的影响。结果1.0×10-7,10-6,10-5和10-4mol/L浓度的辛伐他汀可以抑制患者皮肤成纤维细胞的增殖(P<0.05),并能显著减少成纤维细胞培养液中可溶性胶原的含量,与空白对照组比较,差异有显著性意义(P<0.05),且抑制作用随着药物浓度的增高而增强,具有明显的浓度依赖性。1.0×10-7～10-4mol/L的辛伐他汀可以显著的减少Ⅰ型前胶原蛋白mRNA的表达(P<0.05)。结论辛伐他汀对系统性硬皮病患者皮肤成纤维细胞的增殖及胶原的分泌均有显著的抑制作用,对细胞Ⅰ型前胶原蛋白mRNA的合成具有抑制作用,其为治疗硬皮病提供了理论基础。Objective To study the effects of simvastatin on the biological proliferation, collagen synthesis and type Ⅰ procollagen mRNA expression of human skin fibroblasts of systemic sclerosis. Methods The potential of cell proliferation induced by simvastatin at different concentrations was detected by MTT method. Level of the collagen secretion was detected by bio-chemical method. RT-PCR was used to detect the type I procollagen mRNA expression in the fibroblasts after treatment by simvastatin. Restilts After the treatment by simvastatin in a range of 1.0×10^-7 - 10^-4 mol/L, the proliferation and the collagen synthesis of the cultured dermal fibroblasts of See was inhibited in a dose-dependent manner. There was a significant difference when compared with that of the control （ P 〈 0.05 ）. The level of type Ⅰ procollagen mRNA expression in the fibroblasts was also inhibited by simvastatin. Conclusion Simvastatin can not only inhibit proliferation and collagen secretion of the skin fibroblasts, but also reduce the expression of type Ⅰ procollagen.

关 键 词：硬皮病 系统性 辛伐他汀 成纤维细胞 

分 类 号：R593.25[医药卫生—内科学]