SLE患者GATA-3、MBD2对IL-4表达水平的调控作用  

The Regulation of IL-4 Expression Levels by GATA-3 and MBD2 in Systemic Lupus Erythematosus Patients

作　　者：秦海红[1] 褚国弟[1] 黄峥烨[2] 徐金华[3] 施若非[4] 施伟民[5] 

机构地区：[1]上海市第一人民医院宝山分院皮肤科,上海200940 [2]上海市皮肤病性病医院,上海200050 [3]复旦大学附属华山医院皮肤科,上海200040 [4]上海交通大学附属瑞金医院皮肤科,上海200025 [5]上海同济大学附属同济医院皮肤科,上海200065

出　　处：《中国皮肤性病学杂志》2009年第8期481-483,共3页The Chinese Journal of Dermatovenereology

基　　金：上海市科委基金(064119529)

摘　　要：目的分析SLE患者GATA-3、MBD2与IL-4表达的相关性,探讨GATA-3和MBD2对IL-v4表达的调控作用。方法应用实时定量PCR法检测35例SLE患者和20例正常人外周血单核细胞(PBMC)中IL-4、GATA-3、MBD2的mRNA表达水平,并分析它们之间的相关性。结果SLE患者外周血单核细胞中IL-4、GATA-3和MBD2的表达水平均明显高于正常对照组(t=3.37,3.55,9.68,P均<0.05);IL-4与GATA-3、IL-4与MBD2的表达水平均呈正相关(r=0.39,0.97,P均<0.05)。结论SLE患者中IL-4表达水平升高可能是由于MBD2对IL-4基因阻遏作用降低,及大量GATA-3对IL-4基因的过度激活共同作用所导致。Objective To analyze correlation between GATA-3, MBD2 and to IL-4 gene expression, and to investigate the effect of GATA3 and MBD2 on IL-4 gene expression in SLE patients. Methods The transcription levels of IL-4, GATA-3, MBD2 were detected by using RT-qPCR and correlations were conducted by correlation analysis using spss 11.5 software. Results The expression levels of IL-4,GATA-3 and MBD2 in SLE patients were significantly increased, and there was statistiee3 difference between patients with SLE and normal controls（ t = 3.37, 3.55, 9.68, all P 〈 0.05, ）. There was positive correlation between IL-4 and GATA-3, IL-4 and MBD2 （ r = 0. 39,0. 97, all P 〈 0.05 ）. Conclusion The high expression levels of IL-4 may be caused by loss of gene-slience function of MBD2 and excessive activation of GATA-3 in SLE patients.

关 键 词：红斑狼疮 系统性 IL-4 GATA-3 MBD2 

分 类 号：R593.241[医药卫生—内科学]