纳米Cu^2＋／TiO2抗菌膜对禽流感病毒(H9N2)的灭活效应  

Inactivation efficiency of nano-Cu^(2+)/TiO_2 on avian influenza(H_9N_2)

作　　者：姜建芳[1] 崔海信[1] 杨涛[2] 蔡鸿昌[1] 吴东来[2] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,农业部农业环境与气候变化重点实验室,北京100081 [2]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,农业部兽医公共卫生重点开放实验室,黑龙江哈尔滨150001

出　　处：《功能材料》2009年第8期1403-1406,共4页Journal of Functional Materials

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA10A203,2008AA100803)

摘　　要：利用病毒滴度测定和MDCK细胞电镜观察法,初步探讨了在365nm的黑光灯(UV)照射下,纳米Cu2+/TiO2抗菌膜对禽流感病毒(H9N2)的光催化灭活效应,并分别考察了UV强度、UV照射时间以及H9N2病毒量对H9N2病毒光催化灭活效应的影响。实验结果表明,在365nm黑光灯的照射下,纳米Cu2+/TiO2抗菌膜对H9N2病毒具有显著的灭活效应,在UV强度为0.5mW/cm2、UV照射时间为2.5h、病毒量为0.1ml时,H9N2病毒的灭活率达到了100%。研究结果表明,纳米Cu2+/TiO2抗菌膜在抑制禽流感(H9N2)病毒在环境媒介中的扩散与传播方面有潜在的应用价值。Nano-Cu^2＋/TiO2 was synthesized through a sol-gel method, and its photocatalysis of inactivation efficiency on avian influenza （H9N2） were evaluated under 365nm UV black light. Hemagglutination tests were used to determine the change of the titer of H9N2. Microscope was used to observe the deformation of MDCK cells. The inactivation efficiencies of nano-Cu^2＋/TiO2 sol on H9N2 virus were studied by adjusting the UV intensity, the UV illumination time, and the quantity of H9N2. The results show that nano-Cu^2＋/TiO2 has a remarkable inactivation on H9N2 virus under 365nm UV irradiation. Its inactivating rate on H9N2 viruses can be reached up to 100%, when the UV intensity is 0.5mW/cm^2, the quantity of H9N2 is 0. 1ml, and the UV illumination time is 2.5h. This suggested that the nano-Cu^2＋/TiO2 sol has great potential as a kind of anti-virus agent for preventing Avian Influenza.

关 键 词：Cu^2+/TiO2 禽流感病毒(H9N2) 光催化 灭活效应 

分 类 号：S855.3[农业科学—临床兽医学] X703[农业科学—兽医学]