大鼠心肌缺血再灌注损伤模型的建立与评价  

Establishment and Evaluation of Myocardial Ischemia Reperfusion Injury Model in Rats

作　　者：周学武[1] 廖自福[1] 陈菁[1] 刘良明[1] 

机构地区：[1]创伤、烧伤与复合伤国家重点实验室,第三军医大学大坪医院野战外科研究所二室,重庆400042

出　　处：《实验动物与比较医学》2009年第4期228-232,共5页Laboratory Animal and Comparative Medicine

基　　金：国家重点基础研究发展计划（973计划）资助项目（2005CB522601）.

摘　　要：目的采用左冠状动脉结扎法，建立和评价大鼠心肌缺血再灌注损伤模型。方法麻醉大鼠21只，开胸，穿线结扎左冠状动脉造成缺血45min、松解造成再灌注180min；记录标准Ⅱ导联心电图、MAP和心肌收缩功能、心肌缺血／坏死面积以及大鼠存活时间变化。结果左冠状动脉结扎后，QRS波增宽、增高，ST段上移，T波增高，与缺血前有非常显著差别（P〈0．01）；再灌注后，QRS波宽和波高、ST段和T波非常显著下降（P〈0．01），但仍高于缺血前水平。与缺血前比较，缺血后MAP、HR、LVSP、＋dp／dtmax、-dp／dtmax非常显著下降（P〈0．01），LVEDP非常显著升高（P〈0．01），再灌注后变化趋势相同。180min后，缺血区／左室、坏死区／缺血区的的平均面积比分别为34．3％、49．6％，存活率为52．4％。结论采用左冠状动脉结扎法，复制大鼠心肌缺血再灌注损伤模型，心电图、MAP和心肌收缩功能、心肌缺血／坏死变化明显，成功率较高，是一种较好的建模方法。Objective To establish and evaluate myocardial ischemia reperfusion injury of rats by using left coronary artery ligation. Methods The anesthetized rats of twenty-one were chest-opened. The left coronary artery was ligated with silk thread to produce ischemia of 45 minutes and was then relaxed to induce reperfusion of 180 minutes. The standard II lead of electrocardiogram was recorded. The mean arterial pressure and myocardial contractile function were measured.The average areas of myocardial ischemia/necrosis and survival rate of rats were calculated. Results The width and height of QRS wave were increased （P〈0.01） after ischemia and were then decreased （P〈0.01） significantly after reperfusion. The ST segment and T wave were up-shifted （P〈0.01） after ischemia and were then lowered （P〈0.01） obviously after reperfusion. MAP, HR, LVSP, ＋dp/dtmax and -dp/dtmax were significantly reduced （P〈0.01） and LVEDP was remarkably increased （P〈0.01） after ischemia. The tendency of the above indices after reperfusion was the same as that ofischemia. At 180 minutes, the average area ratio ofischemic zone/left ventricle and necrotic zone/ischemic zone were 34.3% and 49.6% respectively, and the survival rate of rats was 49.6%. Conclusions It was an available method to establish myocardial ischemia reperfusion injury model in rats by using left coronary artery ligation. The changes of electrocardiogram,MAP and myocardial contractile function,as well as myocardial ischemia/necrosis were obvious. The successful rate was achieved.

关 键 词：心肌 缺血再灌注损伤 模型 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]