IFN-γ对妊娠早期大鼠下丘脑-垂体-卵巢轴中IGF-1表达的影响  

Effects of IFN-γ on the expression of IGF-1 in Hypothalamus-Pituitary ovary-Gonad axis of early pregnant rats

作　　者：司丽芳[1,2] 范光丽[1] 赵瑾 朱灿鑫 雷林滨[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]河南科技大学动物科技学院,河南洛阳471002 [3]上海朝翔生物技术有限公司,上海201609 [4]河南夏邑畜牧局,河南夏邑476443

出　　处：《西北农林科技大学学报（自然科学版）》2009年第7期1-5,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(39670550);西北农林科技大学研究生科技创新基金项目(05ych020)

摘　　要：【目的】确定干扰素γ(IFN-γ)对妊娠早期大鼠下丘脑-垂体-卵巢轴中IGF-1表达的影响。【方法】将妊娠9 d的SD大鼠随机分为对照组、试验1组和试验2组,采用生殖道肌肉注射的方法,对照组大鼠生殖道肌肉注射生理盐水,试验组大鼠注射IFN-γ,其中1组剂量为2.5×104U/只,2组剂量为7.5×104U/只。各试验组均在注射48h后灌注取材、免疫组化SP法染色,光镜下观察。【结果】注射2.5×104U/只和7.5×104U/只的IFN-γ均能下调妊娠大鼠下丘脑室周核、视上核、视前大细胞核、视前内侧核、视前外侧核、视前交叉上核、弓状核,腺垂体,卵巢黄体及子宫蜕膜基质细胞和子宫腺中IGF-1的表达。【结论】IFN-γ对妊娠早期大鼠下丘脑-垂体-卵巢轴中IGF-1的表达具有下调作用,高剂量IFN-γ的下调作用更显著。[Objective] The study investigated the effect of interferon-γ（IFN-γ）on the expression and distribution of insulin-like growth factor-1（IGF-1 ）immuonorea-etive production in Hypothalamus-Pituitary ovary-Gonad axis of earlier pregnant rats in the post-implantation period. [Method] In this study the ultrasensitive immunohistochemistry SP method was used 9 d pregnant rats were divided into control group, experimental group one and experimental group two. The control group was injected physiological brine and the two experiment groups were injected 2.5 ×10^4 U and 7.5 ×10^4 U of IFN-γ in vaginal muscular respectively. Experiment materials were collectd after perfusion from each group which was injected 48 h ago,then stained by ultrasensitive immunohistochemistry SP method. And the results were observed and analyzed. [Result] The expressions of IGF-1 in pre-opticus medianus, nucleus supra-opticus, nucleus preopticus mango cellularis, nucleus pre-opticus medialis,mucleus pre-optieus lateralis, nucleus pre-opticus suprachiasmatic and arcuate nucleus of the hypothalamus, adenohypophysis,corpus luteum of ovary, stroma cells of uterus decidua and uterine gland were reduced after being injected 2.5 ×10^4 U and 7.5 ×10^4 U IFN-γ. [Conlusion] The results suggested that IFN-γ could reduce the expression of IGF-1 immunological reaction production in Hypothalamus-Pituitary gland-Gonad axis. High dose of IFN-γ showd a much more significant down regulation.

关 键 词：胰岛素样生长因子-1 干扰素-Γ 免疫组化 下丘脑-垂体-卵巢轴 妊娠大鼠 

分 类 号：S859.797[农业科学—临床兽医学]