响应面法优化Xenorhabdus nematophila发酵培养基的研究  

Enhanced antibiotic activity of Xenorhabdus nematophila by fermentation medium optimization with response surface methodology

作　　者：王永宏[1] 张强[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第7期140-146,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"计划项目(2003AA241140);西北农林科技大学青年学术骨干项目(52211241);西北农林科技大学科研专项

摘　　要：【目的】提高Xenorhabdus nematophilaYL001的抗菌活性,为该菌株杀菌活性成分的提取分离及生物农药的开发应用奠定基础。【方法】以YSG培养基为基础,采用单因子试验方法,对最佳碳源和氮源进行筛选,并采用全因子中心组合试验设计和响应面法对其最佳配比进行优化。【结果】YL001菌株的最佳碳源为玉米粉,氮源为豆饼粉,培养基的最佳组成为:玉米粉9.69 g/L,豆饼粉76.50 g/L,MgSO41.07 g/L,(NH4)2SO41.79 g/L,KH2PO40.63 g/L,K2HPO40.80 g/L,Na2SO41.25 g/L,在此条件下,YL001菌株抗菌活性达268.9 U/mL。【结论】培养基优化后YL001菌株的抗菌活性与试验的预测值接近,表明响应面法在培养基优化中十分有效,相对简单,且节省时间和材料。[Objective] The study enhanced the antibiotics activity of Xenorhabdus nematophila YL001,and laid foundation for antibacterial active constitutents separation of YL001 strain,development and application of such biological resource. [Method] YSG was employed as an original medium, and the best carbon and nitrogen sources were identified using one-factor-at-a-time approach. A full factorial composite experimental design and response surface methodology were used in the design of experiments and in the analysis of results. [Result] Corn flour and bean cake powder were identified as the best carbon. The optimal levels of medium components were：corn flour 9.69 g/L,bean cake powder 76.50 g/L,MgSO4 1.07 g/L, （NH4）2SO4 1.79 g/L,KH2PO4 0.63 g/L,K2 HPO4 0. 80 g/L,Na2SO4 1.25 g/L. [Conclusion] The results strongly support the use of RSM for medium optimization. The chosen method of optimization of medium composition was efficient,relatively simple and time and material saving.

关 键 词：XENORHABDUS nematophila 抗菌活性 响应面法 发酵培养基 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治]