重组人骨形态发生蛋白诱导异位成骨的病理学观察  

Pathological study on ectopic bone formation induced by recombinant human bone morphogenetic protein 2 in mice

作　　者：李双良[1,2,3] 赵明[1,2,3] 董罡[1,2,3] 李春海 乔伯英[1,2,3] 陈华[1,2,3] 赵武宪 

机构地区：[1]解放军总医院医学实验动物中心 [2]军事医学科学院基础医学研究所 [3]解放军总医院病理科

出　　处：《军医进修学院学报》1998年第3期210-212,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的：本文报告了重组人骨形态发生蛋白－２（ｒｈＢＭＰ－２）在小鼠肌肉内诱导异位成骨的生物学作用。方法：将大肠杆菌表达的ｒｈＢＭＰ－２成熟肽植入小鼠股部肌肉。结果：植入ｒｈＢＭＰ－２后ｄ１就有间充质细胞向植入区内聚集；ｄ５出现典型的软骨细胞；ｄ７钙化开始；ｄ１０形成雏形的骨髓腔；ｄ１４骨髓腔内出现幼稚的造血细胞；２１ｄ～３０ｄ形成大量网状骨小梁、骨单位，在骨小梁间隙出现骨髓细胞。Objective:In order to test the biological activity of recombinant human bone morphogenetic protein 2 (rhBMP2).Methods:Affter injection of rhBMP2 into the mouse muscles, the differentiation of mesenchymal cells and timecourse of bone formation was studied during the ectopic bone formation.Results:The rhBMP2 expressed by E. coli presented great boneinduction activity. 1 day after implantation of rhBMP2, the mesenchymal cells from muscular loose connective tissues and blood vessels concentrated in the implantation area. On day 5 typical cartilage tissue and chondrocytes appeared. Calcification emerged on day 7. Infantile hematopoietic cells became visible intramedullaryly on day 14, and a large number of reticular bone trabecula and osseous tissues formation and bone marrow cells emerged from bone trebecula gap by day 21￣30. Conclusion:The endochondral ossification was the main pattern of bone formation induced by rhBMP2. Our results indicate that rhBMP2 can be replaced in the repair of bone defect and treatment of fracture.

关 键 词：重组 骨生成 病理学 骨形态发生蛋白 异位成骨 

分 类 号：R680.2[医药卫生—骨科学]