表征发酵产氢活性污泥效能的碘硝基四唑紫-比脱氢酶活性及其检测  

Detection of Specific Dehydrogenase Activity for Evaluating the Hydrogen-producing Activity of Flocs in Anaerobic Fermentation Process

作　　者：李建政[1,2] 王硕[1,2] 郑国臣[1,2] 蒋凡[1,2] 

机构地区：[1]哈尔滨工业大学 [2]城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《科技导报》2009年第15期104-108,共5页Science & Technology Review

基　　金：中国高技术研究发展计划(863计划)项目(2006AA05Z109);哈尔滨市科技创新人才研究专项(2009RFXXS004)

摘　　要：目前,评价有机废水发酵法生物制氢系统产氢效能的指标,一般采用挥发性悬浮固体(MLVSS)与总悬浮固体(MLSS)的比值(MLVSS/MLSS)、MLVSS的比产气(氢)速率及COD去除率等参数,但均存在不同程度的缺陷。为寻求评价发酵制氢活性污泥系统产氢效能的合理指标,以脱氢酶检测的碘硝基四唑紫(INT)法为基础,以发酵产氢系统的絮状活性污泥为样品,确立并优化了INT-比脱氢酶活性的检测与计算方法,并对比脱氢酶活性与发酵制氢系统的产氢效能进行了相关性分析。结果表明,INT-比脱氢酶活性检测的适宜条件是:在容积为10mL的离心管中,先后加入一定浓度的污泥样品0.3mL、19.8mmol/LINT溶液1mL、pH5乙酸钠缓冲剂1.5mL,45℃暗处振荡反应30min;反应终止剂选用98%的浓硫酸,萃取溶剂为无水乙醇,438nm测定吸光度;检测污泥样品(MLVSS,挥发性悬浮固体)浓度可以在3.5～12.5g/L范围内选定。絮状发酵产氢活性污泥的INT-比脱氢酶活性与比产氢速率非常高度相关,可以客观准确地反映厌氧生物制氢系统污泥的产氢活性。The ratio between Mixed Liquor Volatile Suspended Solid （MLVSS） and Mixed Liquor Suspended Solid （MLSS）, the specific hydrogen producing rate by MLVSS and the COD removal rate are normally used to evaluate the hydrogen-production efficiency of anaerobic activated sludge in fermentation processes, but they suffer from some drawbacks. In order to evaluate the hydrogen-production efficiency of anaerobic activated sludge in fermentation processes, iodonitrotetrazoliumchloride a （INT） detection method using is proposed and optimized for detecting the specific dehydrogenase activity in the flocs. The correlation between dehydrogenase activity and the specific hydrogen production rate of the activated sludge is analyzed. The results show that the optimal conditions for detecting INT- dehydrogenase activity are as follows. A 2.8 mL reaction system, including 0.3 mL sludge sample with a mixed liquor volatile suspended solids of 3.5 - 12.5 g/L, 1 mL 19.8 μmol/L INT and 1.5 mL sodium acetate buffer with a pH of 5, is put in dark for 30 min under 45℃ with oscillation. Concentrated sulfuric acid of 98% is used as the reaction termination agent, while the anhydrous ethanol is used as the extraction solvent. The absorbance is measured at 438 nm. The dehydrogenase activity of the activated sludge is very highly correlated with its specific hydrogen production rate, excellently reflecting the hydrogen-producing activity.

关 键 词：生物制氢 发酵 厌氧活性污泥 脱氢酶 碘硝基四唑紫 

分 类 号：X703[环境科学与工程—环境工程]