电针任脉经穴对脑缺血再灌注大鼠脑内bFGF蛋白及bFGFm RNA表达的影响  

Effects of Electroacupuncturing Ren Vessel on Protein and mRNA Expression of bFGF After Focal Cerebral Ischemia-Reperfusion in Rats

作　　者：杨卓欣[1] 马晓明[2] 于海波[1] 吴志强[1] 皮敏[1] 王玲[1] 郑晓斌[2] 

机构地区：[1]广州中医药大学附属深圳市中医院,广东深圳518033 [2]广州中医药大学,博士研究生广东广州510405

出　　处：《中华中医药学刊》2009年第8期1580-1583,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省自然科学基金资助项目(5009688)

摘　　要：目的:观察电针任脉经穴对脑缺血再灌注大鼠脑内碱性成纤维细胞生长因子(basic fibrob lasticgrowth factor,bFGF)蛋白及mRNA表达的影响。方法:采用线栓法建立大鼠大脑中动脉栓塞局灶性脑缺血再灌注模型。72只实验动物随机分为假手术组(Sham)、脑缺血再灌注模型组(MCAO)、模型+电针任脉治疗组(R)。用免疫组化及原位杂交的方法检测脑内侧脑室下区、海马齿状回区bFGF蛋白及bFGFmRNA的阳性表达。结果:模型组与假手术组相比,bFGF蛋白及bFGFmRNA的阳性细胞数均有不同程度的增加,其中造模后7天、14天差异有统计学意义(P<0.05);电针任脉组的阳性表达多于模型组,但于造模后7天差异无统计学意义(P>0.05),造模后14天及28天差异均有统计学意义(P<0.05)。结论:电针任脉经穴能够上调脑缺血再灌注大鼠脑内bF-GF蛋白及bFGFmRNA表达,这可能是电针任脉促进脑缺血损伤后神经修复的保护机制之一。Objective ：To observe the effects of electroacupuncturing ten vessel on protein and mRNA expression of bFGF after focal cerebral ischemia-reperfusion in rats. Methods ： Cerebral ischemia - reperfusion injury model was induced by the method of nylon monofilament embolism. 72 rats were randomly divided into sham operation group（Sham） ,ischemia - reperfusion model group （MCAO） and ischemia-reperfusion injury with electroacupuncturing ren vessel treatment group （R）. Protein and mRNA expression of bFGF in subventricular zone（SVZ）and Dentate Gyrus（ DG）were observed by immunohistochemistry and in situ hybridization. Results ： Compared with Sham group, more positive cells of bFGF protein and bFGFmRNA could be observed in MCAO group. And there are statistic difference at 7 days and 14 days after cerebral ischemia-reperfusion（ P 〈 0. 05）. More positive cells could be seen in R group than those in MCAO group, but no statistic difference betwwen MCAO-7 group and R-7 group（ P 〉 0.05 ）. There was statistic difference between MCAO and R group at 14 days and 28 days after cerebral ischemia-reperfusion（ P 〈 0. 05）. Conclusion：Electroacupuncturing Ren Vessel can enhance protein and mRNA expression of bFGF,which might be one of the mechanisms of electroacupuncturing Ren Vessel＇s protection against cerebral ischemla-reperfusion injury. Key words： electroacupuncturing ten vessel;cerebral ischemia-repeffusion; bFGF

关 键 词：电针任脉 脑缺血再灌注 BFGF 

分 类 号：R245.9[医药卫生—针灸推拿学]