软脉灵口服液对拟血管性痴呆大鼠学习记忆及海马自由基代谢的影响  

Effect of Ruanmailing Koufuye on Learning and Memory and the Radical Metabolism in Hippocampal Tissue in Rats with Vascular Dementia

作　　者：黄俊山[1] 张维波[2] 林求诚[1] 郑兴敏[2] 林坚[2] 李璟怡[3] 张作丹 

机构地区：[1]福建省中医药研究院,福建福州350003 [2]福州市第一医院,福建福州350009 [3]福建中医学院,福建福州350108 [4]泉州医学高等专科学校,福建泉州362000

出　　处：《中华中医药学刊》2009年第8期1734-1737,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：福州市科技计划社会发展资助项目(2007-s-125)

摘　　要：目的:观察软脉灵口服液对实验性血管性痴呆(VaD)大鼠空间学习记忆能力和自由基代谢的影响。方法:选用永久性结扎双侧颈总动脉(2VO)制作VaD模型,用Morris水迷宫测定大鼠学习记忆能力,比色法大鼠海马组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果:与模型组比较,软脉灵高剂量组逃避潜伏期缩短(P<0.001)、1min穿越原平台次数增加(P<0.001)、海马组织中SOD活性及GSH含量增加(P<0.001,P<0.01)、MDA含量下降(P<0.001);软脉灵低剂量组大鼠海马组织中SOD活性比模型组增加(P<0.01)、MDA含量下降(P<0.05);与软脉灵低剂量组比较,高剂量组大鼠海马组织中GSH含量更高(P<0.05),MDA含量更低(P<0.05)。结论:软脉灵口服液能改善拟VaD大鼠的空间学习记忆能力,减少拟VaD大鼠海马氧自由基堆积。这些作用呈一定的量效关系。Objective ：To study the effect of ruanmailing koufuye on learning and memory and the radiclal metabolism in hippocampal tissue in rats with vascular dementia. Methods ： We established a Vad rat model after chronic cerebral hypoperfusion caused by permanent bilateral common carotid arteries occlusion（ 2VO）, Morris water maze experiment was adopted to test learning and memory in rats of each group. Colorimetric method was used to detect the SOD activity, the MDA and GSH content in the hippocampal tissue of rats. Results：Escape latency was significantly shortened and the number of entries into the target area was significantly increased of rats in high dose group compared with that in model group of rats（ P 〈 0. 001 ）. SOD activity and GSH content in the hippocampal tissue was significantly increased while MDA content was decreased remarkabaly in rat of high dose group compared with that in model group（P 〈0. 001 or P 〈0. 001 ）. Compared with the model group, SOD activity in the hippocampal tissue was significantly increased while MDA was decreased in rats of low dose group （ P 〈 0.01 or P 〈 0. 05 ）. Compare with the low dose group, GSH content in the hippocampal tissue was higher and MDA content was lower in rats of high dose group （ P 〈 0. 05 ）. Conclusion ： Ruanmailing koufuye improve the learning and memory in rats with vascular dementia, reduce the accumulation of oxygen free radial, all these effects were in effect-dose manner.

关 键 词：软脉灵口服液 血管性痴呆 学习记忆 氧自由基 

分 类 号：R285.5[医药卫生—中药学]