银杏内酯B协同地塞米松对小鼠哮喘模型气道炎症和辅助T细胞亚群影响作用的研究  

Effects of Ginkgolide B Coordinated with Dexamethasone on Allergic Airway Inflammation and T Helper Cell Subsets in Asthmatic Rats

作　　者：吕进泉[1] 张晓鸣[1] 吕剑平[1] 忻悦[1] 唐燕[1] 

机构地区：[1]江苏大学附属医院儿科,江苏镇江212001

出　　处：《中国现代医生》2009年第23期25-26,共2页China Modern Doctor

基　　金：江苏省科技厅立项课题(BS2003643)

摘　　要：目的探讨银杏内酯B协同地塞米松对支气管哮喘气道变应性炎症及辅助T细胞亚群的作用。方法40只BALB/c小鼠随机分成正常对照组、哮喘对照组、地塞米松组、银杏内酯B组和联合干预组,每组8只。收集支气管肺泡灌洗液(BALF),行白细胞(WBC)及嗜酸性粒细胞(EOS)计数;应用ELISA法测定BALF中IFN-γ和IL-4水平。结果BALF中WBC及EOS计数:3个用药组较哮喘对照组明显减少(P<0.01),联合干预组减少的程度大于地塞米松组(P<0.05)、银杏内酯B组(P<0.01)。3个用药组小鼠BALF上清中IFN-γ和IL-4水平与哮喘对照组比较,银杏内酯组、地塞米松组和联合干预组BALF上清中IFN-γ和IL-4水平分别增高和降低(P<0.05或P<0.01),而联合干预组增高和降低的程度大于地塞米松组(P<0.01)、银杏内酯组(P<0.05)。结论银杏内酯B能减轻小鼠哮喘模型BALF中炎性细胞浸润,并减少IL-4的水平;银杏内酯B与激素具有协同抗炎作用;同时具有更好的调节Th亚群失衡的作用。Objective To investigate the effects of Ginkgolide B（ GB ）coordinated with Dexamethasone（DXM）on allergic airway inflammation and T helper cell subsets in asthmatic rats. Methods Forty BALB/c rats were randomly divided into 5 groups equally：the normal group, the asthma model group,the DXM group,the GB group and the DXM ＋ GB group. The WBC count and eosinophil（EOS）count in bronchoalveolar lavage fluid（BALF） were estimated and ELISA was used to determine the concentrations of IFN-γ and IL-4 in BALF. Results Compared with the asthma model group,EOS and WBC count and IL-4 level in BALF were significantly lower in the three treated groups （P〈 0.01）,particularly in the DXM ＋ GB group,showing a significant difference as compared with the other two groups（P〈0.05 or P〈0.01 ）, whereas IFN-γ level was increased. And in the DXM ＋ GB group, they showed a significant difference as compared with the other two groups （P〈 0.05 or P〈 0.01 ）. Conclusion Gingkgolide B has an inhibitory effect synergistic with Dexamethasone in airway inflammation,and might partly reverse T helper cell subsets in rats with asthma.

关 键 词：支气管哮喘 银杏内酯B 气道炎症 T辅助细胞亚群 

分 类 号：R969[医药卫生—药理学]