赤眼蜂种间沃尔巴克氏体水平人工转染供体蜂种的筛选  

Screening of the Trichogramma Species for Horizontal Transfer of Wolbachia Symbionts between Trichogramma Wasps

作　　者：张海燕[1] 王倩[1] 付海滨[2] 丛斌[1] 董辉[1] 解焱艳[1] 姬景红[3] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]沈阳出入境检验检疫局,沈阳110016 [3]黑龙江省农业科学院土壤肥料与环境资源研究所,哈尔滨150086

出　　处：《中国生物防治》2009年第3期281-284,共4页Chinese Journal of Biological Control

基　　金：国家"十一五"科技支撑计划(2006BAD08A06);国家自然科学基金项目(30771455)

摘　　要：实验室条件下以感染沃尔巴克氏体的引进种卷蛾赤眼蜂、食胚赤眼蜂、短管赤眼蜂为材料,比较分析3种赤眼蜂连续3代喂食抗生素后子代雄蜂率、寄生和羽化情况。除卷蛾赤眼蜂F_2代外,取食四环素后3种赤眼蜂各世代寄生率均降低,其中食胚赤眼蜂的寄生率与对照相比明显下降;3种赤眼蜂间寄生率没有明显差异。随着取食抗生素世代的增加,3种赤眼蜂子代雄蜂率逐渐增大,短管赤眼蜂F_1代开始出现雄蜂,F_3代雄蜂率高达54.54%,明显高于卷蛾赤眼蜂(20.58%)和食胚赤眼蜂(13.94%);食胚赤眼蜂3个世代羽化雄蜂率均最低,说明其产雌孤雌生殖方式比较稳定,较适合作为赤眼蜂种间沃尔巴克氏体水平人工转染的供体。In laboratory,effects of feeding tetracycline on offspring male ratio and parasitism were investigated on three introduced Trichogramma species,T.cacoeciae Marchall,T.embryophagum Hartig and T.pretiosum infected by Wolbachia.After antibiotic treatment,parasitism of offspring of the three Trichogramma species decreased,and significant decrease occurred in T.cacoeciae in comparison with the control.No difference in parasitism was detected between the three Trichogramma species except F2 of T.cacoeciae.Male ratio increased in the three Trichogramma species when the wasps were fed antibiotics on successive generations.Male offspring appeared at F1 generation in T.pretiosum,reached 54.54%of the total emerged wasps at F3,which was higher than those in T.pretiosum（20.58%） and T.cacoeciae（13.94%）.It is concluded that T.embryophagum,which showed stable thelytokous parthenogenesis,can be used as donator of Wolbachia in horizontal transfer between species.

关 键 词：沃尔巴克氏体 赤眼蜂 产雌孤雌生殖 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] Q939.3[农业科学—植物保护]