环介导等温扩增快速检测阳性血培养瓶中的金黄色葡萄球菌  

Rapid Identification of Staphylococcus aureus in Positive Blood Culture Bottles by Loop-mediated Isothermal Amplification

作　　者：周义正[1] 王昌富[1] 李向阳[2] 邱小燕[1] 付千钧[1] 杨锦红[2] 

机构地区：[1]荆州市中心医院检验科,湖北荆州434020 [2]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《中华医院感染学杂志》2009年第16期2075-2077,共3页Chinese Journal of Nosocomiology

摘　　要：目的建立采用环介导等温扩增(LAMP)技术从阳性血培养瓶中快速检测金黄色葡萄球菌的方法。方法以293份阳性血培养瓶中的细菌为检测对象,采用盐酸胍-苯甲醇法提取细菌DNA,然后以LAMP技术扩增ssa和mecA基因来鉴定金黄色葡萄球菌及其对甲氧西林的耐药性,并将检测结果与传统方法相比较。结果在293份阳性血培养瓶中,采用LAMP法检测到22株金黄色葡萄球菌,与传统方法比较,其检测金黄色葡萄球菌的灵敏度和特异度均达100%,且检测时间缩短约1 h。结论该方法灵敏度高、特异性强、检测方便快速,适合从阳性血培养瓶中快速检测金黄色葡萄球菌。OBJECTIVE To establish a method of loop-mediated isothermal amplification（LAMP） for detecting Staphylococcus aureus in positive blood culture bottles. METHODS Genomic DNA in 293 positive blood culture bottles was extracted by guanidine hydrochloride and benzenemethanol, then genes ssa and meeA were amplified by LAMP to identify S. aureus. Finally, the results of LAMP were compared with the results of traditional method. RESULTS Twenty-two strains of S. aureus were detected in 293 positive blood culture bottles by LAMP method. Compared with traditional method, the sensitivity and specificity of LAMP method were both 100%, respectively, the detection could be finished in an hour. CONCLUSIONS The LAMP-based assay is simple, rapid, sensitive and specific which can be used to detect S. aureus in positive blood culture bottles rapidly.

关 键 词：金黄色葡萄球菌 甲氧西林 耐药性 

分 类 号：R378.11[医药卫生—病原生物学]