革兰阳性球菌体外耐药监测与分析  

In Vitro Monitoring and Analysis of Drug-Resistance of Gram-positive cocci

作　　者：李惠[1] 杨玉书[1] 

机构地区：[1]湖北省建始县人民医院检验科,湖北建始县445300

出　　处：《中华医院感染学杂志》2009年第16期2183-2184,共2页Chinese Journal of Nosocomiology

摘　　要：目的探讨革兰阳性球菌(G+球菌)的耐药现状,为临床合理用药提供科学依据。方法336株G+球菌分离自2003年1月-2007年12月医院临床各类感染性标本,G+球菌的培养与鉴定严格按照《全国临床检验操作规程》进行,药物敏感试验采用K-B法。结果336株G+球菌中金黄色葡萄球菌(SAU)居首位,占45.8%;其次为凝固酶阴性葡萄球菌(CNS),占23.2%;粪肠球菌居第3位,占18.2%;耐甲氧西林金黄色葡萄球菌(MRSA)检出率为31.8%;耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)检出率为35.9%;药敏结果显示,G+球菌除对万古霉素耐药率为0以外,对其他常用抗菌药物均产生了不同程度的耐药性。结论各级医院必须重视G+球菌的耐药性检测,预防耐药菌株的传播与流行。OBJECTIVE To investigate drug resistance status of Gram-positive cocci in the local region and offer scientific basis for reasonable usage of antibiotics. METHODS 336 strains of Gram-positive cocci were isolated from all kinds of specimens from Jan 2003 to Dee 2007 in the local hospital. Organisms were cultivated and identified complying with National Operation Procedure of the Clinical Laboratory. Drug sensitivity test was performed with K-B methods. RESULTS Among the 336 strains, Staphylococcus aureus （SAU） ranked the top, accounting for 45. 8%; then the coagulase-negative Staphylococcus （CNS） accounting for 23. 2%; Enterococcus faecalis ranked the third, accounting for 18.2%; the detection rate of meticillin-resistant Staphylococcus aureus （MRSA） was 31.8%; that of meticillin-resistant coagulase-negative Staphylococcus（MRCNS） was 35.9%. The results of drug sensitivity test showed the Gram-positive cocci were resistant to the many antibiotics in different degrees except for 100% sensitive to vancomycin. CONCLUSIONS Hospital should attach importance to supervise the drug resistance of Gram-positive cocci and prevent the dissemination and epidemic of drug resistant strains.

关 键 词：革兰阳性球菌 耐甲氧西林葡萄球菌 耐药性 检测 

分 类 号：R378[医药卫生—病原生物学]