支原体培养及药敏试验结果分析  

Mycoplasma Culture and Analysis of Susceptibility Test

作　　者：陈杨芳[1] 邹珂[1] 

机构地区：[1]温州市第三人民医院检验科,浙江温州325000

出　　处：《中华医院感染学杂志》2009年第16期2208-2209,共2页Chinese Journal of Nosocomiology

摘　　要：目的比较4种品牌支原体液体培养、鉴定、药敏一体化试剂盒检测解脲脲支原体(Uu)及5种抗菌药物药敏试验结果分析,评价不同厂家支原体试剂的应用效果。方法采用法国生物梅里埃公司(MycoplasmaIST2)、珠海丽珠(ICS)、黑马(IES)、上海其昌达等4种支原体检测试剂盒,在相同条件下,对260份临床标本做支原体培养鉴定和药敏试验,比较5种抗菌药物药敏结果一致性程度。结果4种品牌支原体液体培养试剂盒48 h对解Uu的检出率分别是IST2为45.4%I、CS为36.2%I、ES为37.7%、上海其昌达为36.9%。药敏试验结果:IST2中交沙霉素、多西环素、克拉霉素、氧氟沙星和阿奇霉素的耐药率最低,明显优于其他3种试剂结果,不同厂家支原体试剂对5种抗菌药物的耐药率差异有统计学意义。结论临床做支原体培养时,选用支原体液体培养鉴定试剂盒应慎重。OBJECTIVE To compare the detection rates of Ureaplasrna urealyticum （Uu）, by 4 kinds rits and find out the best one. METHODS Using four kinds of mycoplasrna detection kits, namely are French rnycoplasmalST2, Zhuhai Livzon＇s ICS, Dark horse＇s IES and Shanghai Its Praehanda, under the same conditions, the rnycoplasma of the Clinical specimens for 260 cases were tested. RESULTS The detection rate of mycoplasrna （Uu） for 48 hours by four kinds of brands kits about rnyeoplasrna liquid culture was as follows 45.4% for French MycoplasmaIST2, 36.2% for Zhuhai Livzon＇s ICS, 37.7% for Dark horse＇s IES and 36.8% for Shanghai Its praehanda. The results of susceptibility test： the reagent of IST2 had. the lowest resistance to josamycin, doxycycline, elarithromycin, ofloxacin and azithromycin,and had the better results than the other three kinds of reagents. Mycoplasrna reagents form different companies had significant differences in resistance rates to five kinds of antibiotics. CONCLUSIONS The results suggest that when we cultivate clinical rnycoplasma should be careful to select of the mycoplasma liquid culture identification kit.

关 键 词：支原体 解脲脲支原体 药敏试验 耐药率 

分 类 号：R375[医药卫生—病原生物学]