昆虫病原线虫共生菌对水稻恶苗病菌的抑菌活性及其抗逆性的研究  

Antibiotic Activities of Symbiotic Bacteria to Fusarium moniliforme and Stress Tolerance

作　　者：王欢[1] 王鹏[2] 刘彦群[1] 丛斌[3] 董辉[3] 冯帆[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161 [2]辽宁喀左县农村经济发展局综合治理办公室,辽宁喀左122300 [3]沈阳农业大学植物保护学院,辽宁沈阳110161

出　　处：《作物杂志》2009年第4期32-34,共3页Crops

基　　金：国家自然科学基金(30170625);国家"十五"攻关课题(2004BA509B04)资助;辽宁省自然科学基金(20022084);沈阳农业大学青年教师科研基金资助项目(2005002)

摘　　要：利用不同品系昆虫病原线虫的共生菌菌株对水稻恶苗病菌进行抑菌活性测定,得到一株抑菌效果良好的共生菌菌株NC34-1,抑菌圈平均半径为17.33mm,并对高毒力菌株的抗逆性(包括不同温度、紫外照射)进行了初步探索,结果表明,共生菌菌株NC34-1在50℃高温下处理10min,其抑菌活性依旧很高;18W紫外线照射120min对共生菌NC34-1的抑菌活性的影响不明显,于LB培养基、160r/min、28℃培养40h时抑菌效果最佳。In this study,antibiotic activities of 41 strains symbiotic bacteria were examined by bioassays. The highly virulent strain was selected through a large number of bioassays. NC34-1 had high antibiotic activity to Fusarium moniliforme and the radius of inhition zone was 17.33mm. The highly virulent strain had antibiotic activity to Fusarium moniliforme at 50℃ for 10min. The culture broth waved by ultraviolet rays from 10min to 120min,the antibiotic activity was still stable. The antibiotic aeticity was the best when the bacterium was incubated for 40h at 28 ℃ on a rotary shaker at 160r. p. m.

关 键 词：昆虫病原线虫 共生菌 抑菌活性 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]