192株志贺菌属和76株沙门菌属菌群菌型分布及耐药性分析  

Analysis on the serotypes and antimicrobial-susceptibility of Shigella and salmonella spp isolates

作　　者：刘迎春[1] 衣美英[1] 李杰[1] 李囡[1] 贾红兵[1] 李春英[1] 王靖[1] 李建华[1] 刘彤[1] 王振庭[1] 詹同顺[1] 

机构地区：[1]中日友好医院检验科,北京100029

出　　处：《中日友好医院学报》2009年第4期213-215,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的:了解我院志贺菌属、沙门菌属流行菌株类型和药物敏感性特点。方法:应用SS和XLD培养基对我院2007年肠道门诊急性腹泻患者粪便标本进行志贺菌属、沙门菌属分离培养,可疑菌株进行生化和血清学鉴定,并采用K-B纸片法对分离菌株进行药物敏感试验。结果:2007年从约1600份标本中分离出192株志贺菌属,其中福氏志贺菌29株(15%),宋内志贺菌163株(占85%);沙门菌属76株,其中D群54株(71%),优势血清型为杜伯林沙门菌;沙门菌对左氧氟沙星、头孢曲松、氯霉素的敏感性较高,敏感率≥95%,复方新诺明、氨苄西林次之。宋内志贺菌和福氏志贺菌对美国医学实验室抗菌药物敏感试验执行标准(CLSI)[1]中推荐的3种药物中的复方新诺明、氨苄西林敏感率均较低(6.7%～25.8%),而对左氧氟沙星的药敏结果差别也较大:宋内志贺菌的敏感率较高(≥95.7%),而福氏志贺菌的敏感率仅为79.4%。结论:我院2007年肠道门诊就诊患者中,分离志贺菌属以宋内志贺菌为主,福氏志贺菌次之。沙门菌属以D群为优势菌群,优势血清型为杜伯林沙门菌。宋内志贺菌和福氏志贺菌对抗菌药物的药敏结果差别较大,临床医师应根据菌群选择抗菌药物。Objective：To investigate the epidemical serotypes of Shigella,salmoneUa and their antimicrobial resistance characteristics in China-Japan Friendship Hospital in 2007.Methods：The specimens of fecal from the patients with acute diarrhea were collected and cultured with SS and XLD medium for Shigella dysenteriae and Salmonella isolation.Suspicious isolates were identified with biochemical and serological test,Standard K-B filter paper method was conducted on the isolate for antimicrobial sensitivity test.Results：One hundred and ninty two Shigella strains were detected,among which 29 strains （15%）were Shigella and Shigella sonnei had 163 cases （85%）.Salmonella was 76,among which 54 was group D accounting for 71%,and the dominant serotype was S.Dublin.Salmonella had high sensitivity with levofloxacin,ceftriaxone,chloramphenicol and the sensitive rate was over 95% ,and sensitive rate of ceftriaxone,ampicillin took the second place.Shigella sonnei and Shigella flexneri sensitive rates toward cotrimoxazole,ampicillin were relatively, low （6.7%-25.8%）,while susceptibility on levofloxacin was quite different （three drugs are recommended by CLSI）.Sonnei Shigella had higher sensitivity rate,which was over 95.7%,while Shigella flexneri antimicrobial sensitivity rates was 79.4%. Conclusion：The main serotypes of Shigella spp in the hospital was s.sonnei,followed with s.flexneri.The main serogroup of the Salmnella spp isolates was group D,and the dominant serotype was S.dublin.There are obvi- ous difference beteen antimicrobial susceptibility of Shigella sonnei and Shigella flexneri.Clinician should choose different antimicrobial drugs for different bacteria.The antimicrobial resistance monitoring should be strengthened according to the intestinal clinical isolates.

关 键 词：志贺菌属 沙门菌属 血清型 药物敏感性 

分 类 号：R446.5[医药卫生—诊断学]