鲜无蹼壁虎抗肿瘤活性成分生物碱脂质体的研制及其质量评价  

Preparation of fresh Gekko.swinhonis.gunther anti-neoplasm active component alkaloid liposomes and its quality evaluation

作　　者：康建功[1] 张仕状[1] 李真真[1] 李耀辉[2] 

机构地区：[1]潍坊医学院附属医院,潍坊261031 [2]潍坊医学院,潍坊261041

出　　处：《中国新药杂志》2009年第15期1453-1459,共7页Chinese Journal of New Drugs

基　　金：国家自然科学基金项目(30572323)

摘　　要：目的:采用旋转薄膜分散-超声法制备鲜无蹼壁虎抗肿瘤活性成分脂质体,并考察其包封率的影响因素。方法:以凝胶过滤法分离脂质体,于220 nm处测定吸收度,计算包封率,以包封率为测试指标,考察鲜无蹼壁虎抗肿瘤活性成分脂质体的制备方法、药脂比、胆固醇含量、超声时间和超声强度对包封率的影响。结果:采用薄膜分散-超声法、药脂比为1∶10,磷脂-胆固醇比为2∶1,超声时间10 min的条件制备脂质体的包封率较高,可达62.5%,在低温4℃条件下放置4个月,包封率基本没有变化。结论:旋转薄膜分散-超声法制备鲜无蹼壁虎抗肿瘤活性成分脂质体包封率较高,质量比较稳定,重现性好。Objective：To prepare fresh Gekko. swinhonis, gunther anti-neoplasmic activity component extractive liposomes by revolve membrane vacuum evaporation hydration-hypersound method, and to study the influ- ence of different factors on the entrapment efficiency. Methods：Sephadex G-50 was used to separate the free alka- loid component from the liposomes. The entrapment efficiency of alkaloid liposomes was determined at 220 nm. The ratios of drug to phospholipids and phospholipids to cholesterol,hypersound time,hypersound intension were measured. The physieo-ehemical properties of drug in liposome were also investigated. Results ：The entrapped efficiency was 62.5% with the 1：20 ratio of drug to lipids,the 2：1 ratio of phospholipids to cholesterol by using revolve membrane vacuum evaporation hydration-hypersound method. The stability study showed that drug liposome was stable at 4℃ for 4 months. Conclusion：The drug liposome with high entrapped efficiency and stability can be obtained by the optimization of preparation and formulation.

关 键 词：鲜无蹼壁虎抗肿瘤活性成分 脂质体 生物碱 制备工艺 包封率 

分 类 号：R943.4[医药卫生—药剂学] R979.1[医药卫生—药学]