猪小肠抗菌肽对SPF鸡肠道黏膜免疫功能的影响  

Effect of Swine Gut Antimicrobial Peptides (SGAMP) on Intestinal Mucosal Immunity of Specific-pathogen-free (SPF) Chickens

作　　者：姚俊 王德成[2] 佘锐萍[2] 马卫明[3] 

机构地区：[1]云南省畜牧兽医科学院,昆明650224 [2]中国农业大学动物医学院,北京100193 [3]山东农业大学动物科技学院,泰安271018

出　　处：《科学技术与工程》2009年第17期4918-4923,共6页Science Technology and Engineering

基　　金：国家自然科学基金(30471301);云南省农业科技创新工程(2008LA002)资助

摘　　要：60只1日龄的SPF鸡随机分为两组,来评价添加外源性抗菌肽对其肠道黏膜免疫功能的影响。结果表明:1)十二指肠、空肠和回肠内的上皮内淋巴细胞数量从第21日龄起,与对照组相比差异明显(p<0.05)。2)自21日龄起,在抗菌肽处理组可以见到明显的肥大细胞,而在对照组少见(p<0.05)。3)抗菌肽的处理能显著增加各段肠道内杯状细胞的数量(p<0.05)。4)使用抗菌肽处理后,能提高各段肠道的sIgA的表达。添加外源性的抗菌物质,能显著提高SPF鸡的肠道黏膜免疫参数,并且有助于进一步深入了解抗菌肽对肠道黏膜免疫的调控机制。Sixty specific-pathogen-free （SPF） chickens were randomly divided into two groups （30 chickens for each group） to determine the effect of swine gut antimicrobial peptides （SGAMP） on intestinal mucosal immunity. All chickens were raised in negative-pressure isolators and fed the same diet. The results were as follows： （1） In the SGAMP group,the number of mast cell were increased markedly in the duodenum from day 21 to 49 （p 〈 0. 05）, and similar results were observed in the jejunum and ileum after treated with SGAMP （p 〈 0. 05）. However,fewer mast cells were observed in those same tissues in the control. （2） Intraepithelial lymphocytes （IELs） in the duodenum,jejunum and ileum were increased significantly from day 21 to 49 in the SGAMP group compared with the control （p 〈 0. 05 ）. （3） Compared with the control, goblet ceils were increased significantly in duodenum and jejunum from day 35 to 49 （p 〈0. 05） after SGAMP treatment,and in the ileum were increased from day 21 to 49 （p 〈 0. 05 ）. （4） SGAMP upgrade the expression of secretary IgA （slgA） at different sites within the intestinal tract. The results strongly support that SGAMP can enhance the intestinal mucosal immune parameters of SPF chickens. Our research contributes to the further understanding of immunoregulatory mechanisms of intestinal mucosal immunity and the contribution of SGAMP to this process.

关 键 词：抗菌肽 上皮内淋巴细胞 肥大细胞 杯状细胞 分泌型IGA 

分 类 号：R392.1[医药卫生—免疫学]