慢性荨麻疹患者血浆RANTES、嗜酸粒细胞趋化因子、TNF—α与白三烯B4水平的测定  

Detection of plasma RANTES, eotaxin, TNF-α and LTB4 in patients with chronic urticaria

作　　者：史立宏[1] 李晓民 李金勇 牟华光 

机构地区：[1]潍坊医学院山东省应用药理学重点实验室,山东潍坊261042 [2]潍坊市皮肤病医院

出　　处：《中华皮肤科杂志》2009年第8期553-555,共3页Chinese Journal of Dermatology

摘　　要：目的探讨慢性荨麻疹患者血浆中调节激活正常T细胞表达和分泌的细胞因子（RANTES）、嗜酸粒细胞趋化因子（eotaxin）、TNF—α及白三烯B4（LTB4）的水平及其意义。方法对41例慢性荨麻疹患者进行临床评价，按症状积分将病情分为轻型、中型、重型3级。应用咪唑斯汀10mg每日1次，连续治疗4周。采用ELISA法测定20例健康志愿者与41例患者治疗前后血浆中RANTES、eotaxin、TNF-α与LTB4的水平。结果①慢性荨麻疹组血浆中RANTES、eotaxin、TNF-α和LTB4水平分别为（52．5±10．2）μg／L、（58．4±16．1）μg/L、（35．1±9．6）ng／L和（109．4±21．7）ng／L，健康对照组水平分别为（33．7±9．4）μg／L、（48．3±13．6）μg／L、（21．3±8．9）ng／L和（77．8±11．6）ng／L，两组比较差异均有统计学意义，P值分别〈0．01、〈0．05、〈0．01、〈0．01。②血浆RANTES、eotaxin、TNF-α、LTB4水平与临床表现的相关性分析显示：中、重型慢性荨麻疹患者的RANTES、eotaxin、TNF-α与LTB4血浆水平高于轻型患者，差别均有统计学意义，P〈0．05；重型患者的RANTES、eotaxin、TNF-α与LTB4血浆水平较中型患者略有增高，但差异均无统计学意义，P〉0．05。③咪唑斯汀治疗后慢性荨麻疹组血浆中RANTES、eotaxin、TNF—α和LTB4水平较前明显下降，分别为（36．3±8．9）μg／L、（46．3±10．2）μg／L、（23．2±7．5）μg／L和（83．1±14．2）ng／L，与治疗前比较差异有统计学意义，P值均〈0．01，与健康对照组比较P值均〉0．05。结论慢性荨麻疹患者血浆RANTES、eotaxin、TNF-α与LTB4水平升高，并与病情的严重程度呈正相关趋势，咪唑斯汀治疗后血浆中RANTES、eotaxin、TNF—α和LTB4水平较前明显下降，提示RANTES、eotaxin、TNF-α与LTB4在慢性荨麻疹的发病过程中可能起一定作用。Objective To investigate the plasma levels of regulated upon activation normal T cell expressed and secreted （RANTES）, eotaxin, tumor necrosis factor （TNF）-a and leukotriene B4 （LTB4） in patients with chronic urticaria and their roles in the pathogenesis of chronic urticaria. Methods Forty-one patients with chronic urticaria were included into this study along with 20 normal human controls. Patients were graded into three groups, i.e. mild group （n = 11 ）, moderate group （n = 21 ） and severe group （n = 9）, according to their symptom score. All patients were treated with mizolastine 10 mg per day for 4 weeks. ELISA was used to study the plasma levels of RANTES, eotaxin, TNF-α and LTB4 in normal controls and patients before and after treatment. Results The plasma levels of RANTES, eotaxin, TNF-α and LTB4 were （52.5 ± 10.2） g/L, （58.4 ± 16.1） g/L, （35.1± 9.6） ng/L and （109.4 ± 21.7） ng/L, respectively, in untreated patients with chronic urticaria, compared to （33.7± 9.4） g/L, （48.3 ± 13.6） g/L, （21.3 ±8.9） ng/L and （77.8 ± 11.6） ng/L, respectively, in normal controls（P 〈 0.01, 0.05, 0.01, 0.01, respectively）. Increased plasma levels of RANTES, eotaxin, TNF-α and LTB4 were observed in patients with moderate or severe chronic urticaria compared with those with mild chronic urticaria （both P 〈 0.05）, whereas there was no significant difference between patients with severe and mild chronic urticaria （P 〈 0.05）. After treatment with mizolas- tine the plasma levels ofRANTES, eotaxin, TNF-α and LTB4 were （36.3 ± 8.9） g/L, （46.3 ± 10.2） g/L, （23.2 ± 7.5） ng/L and （83.1 ± 14.2） ng/L respectively, significantly lower than those in patients before treatment （all P 〈 0.01 ）, but showed no difference from those in normal controls （all P 〉 0.05）. Conclusions The plasma levels of RANTES, eotaxin, TNF-α and LTB4 are elevated in patients with chronic urticaria, and they exhibits a positive correlation tendency with

关 键 词：荨麻疹 RANTES 趋化因子 嗜酸细胞 肿瘤坏死因子α 白三烯B4 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]