药疹患者Epstein—Barr病毒感染的检测  

Preliminary study on Epstein-Barr virus infection in patients with drug eruption

作　　者：朱桂芝[1] 陈官芝[1] 罗兵[2] 

机构地区：[1]青岛大学医学院附属医院皮肤科,266003 [2]青岛大学医学院微生物教研室

出　　处：《中华皮肤科杂志》2009年第8期556-559,共4页Chinese Journal of Dermatology

摘　　要：目的探讨Epstein—Barr病毒（EBV）感染在药疹发病中的作用。方法应用PCR—Southern杂交技术检测32例药疹患者和30例正常人对照外周血单一核细胞中EBV特异性DNA片段BamHⅠ—W．筛选出EBV阳性标本，对EBV阳性标本进行RT—PCR和Southern杂交，检测EBV即刻早期基因BZLF1的表达；同时采用ELISA检测血清EBV衣壳抗原（VCA）-IgM抗体。结果药疹患者和正常人对照EBVDNA阳性率分别为78．13％（25／32）和10．00％（3／30），药疹患者组明显高于正常人对照组（P〈0．01）；轻型药疹和重型药疹患者阳性率的差异无统计学意义（P=0．104）。25例药疹患者EBVDNA阳性标本中有3例EBV BZLF1 mRNA表达阳性，其中轻型药疹患者1例，重型药疹患者2例。32例药疹患者组中有6例EBV VCA—IgM抗体阳性，轻型和重型药疹患者阳性率差异无统计学意义（P=0．672）；30例对照组均为阴性。结论药疹患者中存在EBV的活动性感染，EBV感染可能是影响药疹发病的环境因素之一。Objective To explore the role of Epstein-Barr virus （EBV） infection in the etiology of drug eruption. Methods PCR-Southem blot was used to detect EBV-specific DNA fragment BamH Ⅰ -W in peripheral blood mononuclear cells of 32 patients with drug eruption and 30 age- and sex-matched normal controls. The mRNA expression of EBV lyric gene BZLF1 in EBV DNA-positive samples was measured by RT-PCR and Southern blot. ELISA was performed to detect EBV virus capsule antigen （VCA）-specific IgM. Results The positivity rate of EBV DNA was significantly higher in patients with drug eruption than in normal controls （78.13% （25/32） vs 10.00% （3/30）, P 〈 0.01 ）, while no significant difference was noted between patients with severe and mild drug eruption （P 〉 0.05）. The expression ofBZLF1 mRNA was detected in 3 out of 25 EBV DNA-positive patients; of the 3 patients, 1 suffered from mild drug eruption, and 2 from severe drug eruption. EBV VCA-specific IgM was observed in 6 of 32 patients with drug eruption, but not in any normal controls. No significant difference in the positivity rate ofEBV VCA-specific IgM existed between patients with severe and mild drug eruption （P 〉 0.05 ）. Conclusions There is an active infection of EBV in patients with drug eruption. EBV infection is probably an environmental factor affecting the development of drug eruption.

关 键 词：药疹 爱泼斯坦巴尔病毒感染 基因 即早 免疫球蛋白M 

分 类 号：R758[医药卫生—皮肤病学与性病学]