静磁场处理液体培养基对三种需氧菌生长的影响  

Effects of Static Magnetic Fields on Aerobes:Escherichia coli,Staphylococcus aureus and Bacillus subtilis

作　　者：靳峰[1] 刘陶[1] 李发荣[1] 何建平[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《生物医学工程学杂志》2009年第4期757-760,共4页Journal of Biomedical Engineering

基　　金：教育部博士点专项基金资助项目(20050698017)

摘　　要：用0.085～0.092T的静磁场处理营养肉汤培养基12h,然后在磁场处理过的培养基中静置培养大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillus subtilis),测定三种细菌在不同生长时间的OD600值。结果显示:与对照组相比,三种需氧菌磁场处理组OD600值在3～9h之间明显高于对照组,差异显著,培养11h后处理组和对照组OD600值则无差异。碘量法测定磁处理组和对照组营养肉汤培养基的溶解氧,处理组比对照组溶解氧含量平均升高15%左右,结果差异有显著性(P<0.05)。以上结果表明:磁场处理液体培养基使培养基中溶解氧含量升高,促进需氧菌对数期生长,磁场对需氧菌的生长的影响可能与培养基中含氧量的变化相关。Nutrient agar medium was exposed in 0. 085-0. 092 T static magnetic fields for 12h. Then we densities the optical densities at λ= 600（OD600 ） of Escherichia coli, Staphylococcus aureus and Bacillus subtilis in different culturing stage. The results were compared with those of control group in the normal geomagnetic field. The OD600 values of experimental groups of these three kinds of aerobes were significantly higher than those of control groups from 3h to 9h. However, after 11 h, there was no remarkable difference regarding the OD600 values between the two groups. The dissolved oxygen content of nutrient agar medium was determined by Micro-titration. The dissolved oxygen of nutrient agar medium under static magnetic for 12h increased 15% in average and there was significant difference when compared with the control. The results showed that the ferro-magnetic fields increased the dissolved oxygen content of nutrient agar medium significantly. These findings suggest that the effects of static magnetic fields on Escherichia coli, Staphylococcus aureus and Bacillus subtilis are related to the dissolved oxygen.

关 键 词：静磁场 溶解氧 需氧菌 

分 类 号：Q937[生物学—微生物学]