工程化人工肌腱玻璃化保存的实验研究  

Study on Cryopreservation of Tissue Engineered Tendon by Vitrification

作　　者：刘程俊[1] 秦廷武[1] 王治[1] 陈曦[1] 杨志明[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室干细胞与组织工程研究室,成都610041

出　　处：《生物医学工程学杂志》2009年第4期847-851,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30570469)

摘　　要：为了摸索工程化人工肌腱玻璃化保存的简易方法,采用3种不同的深低温冻存剂探讨不同的冻存保护剂对工程化人工肌腱的影响。将工程化人工肌腱保存实验分为21%DMSO组、DP6组和VS55组。通过扫描电镜、流式细胞术检测和激光共聚焦显微镜等研究冻存后细胞存活率及在支架材料上的黏附特性。结果显示:DP6组与VS55组之间,细胞存活率无显著差异(P>0.05);而21%DMSO组分别与DP6组、VS55组之间,细胞存活率差异有统计学意义(P<0.05);且21%DMSO组在冻存前后,细胞均与支架材料特异性黏附。提示,在现有实验室操作条件下,21%DMSO比DP6与VS55更适合作为工程化人工肌腱的玻璃化冷冻保存液。In search of a practical method for the cryopreservation of tissue engineered tendon （TET） by vitrification, we adopted 3 kinds of different cryoprotective agents （CPA） （21% DMSO, DP6 and VS55） in studying the freeze-stored effect of different CPA. The cellular morphology and post-thaw viability of the TET were examined by scanning electron microscopy （SEM）, flow cytometry, and confocal laser microscopy （CLM）. The results showed that there existed statistically significant difference in respect to the post-thaw viability between 21% DMSO and DP6, VS55; The cells specially adhered to the surface of scaffold both before or after cryopreservation by use of 21% DMSO. It was suggested that 21%DMSO as a CPA for TET cryopreservation was better than DP6 and VS55 in the current study.

关 键 词：组织工程 肌腱 玻璃化 深低温冻存 冻存保护剂 

分 类 号：R318.17[医药卫生—生物医学工程]