甘草酸二铵磷脂复合物对小鼠慢性肝损伤的保护作用  

Protective effects of diammonium glycyrrhizinate phospholipid complex on chronic liver injury

作　　者：茹仁萍[1] 陈晓瑾[1] 尤琳雅[1] 唐红英[1] 蔡汉萍[1] 匡荣[2] 

机构地区：[1]杭州市第六人民医院药剂科 [2]浙江省食品药品检验所

出　　处：《中国临床药理学与治疗学》2009年第6期642-645,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：杭州市科技局项目(20051333838)

摘　　要：目的:探讨甘草酸二铵磷脂复合物注射剂(DG-PC)对C57BL/6J小鼠慢性免疫性肝脏损害的保护作用。方法:复制C57BL/6J小鼠慢性免疫性肝脏损害模型,测定血清ALT、AST、蛋白和透明质酸(HA),肝匀浆中超氧化物岐化酶(SOD)、谷胱甘肽(GSH)、I型胶原(C.I),并对肝脏组织切片进行电镜观察。结果:DG-PC可以明显改善肝损小鼠AST水平、提高白蛋白(ALB)含量、升高白球比(A/G)、降低HA含量(P<0.01),提高肝损小鼠的SOD和GSH水平(P<0.01),治疗效果优于甘草酸二铵(DG)。小鼠肝组织透射电镜观察显示DG-PC可以保护肝细胞和细胞器,减轻纤维化程度。结论:DG-PC对于C57BL/6J小鼠慢性免疫性肝损伤均具有一定的保护作用,可能与抗氧化等活性有关。AIM： To approach the protective effects of diammonium glycyrrhizinate phospholipid complex injection （DG-PC） on chronic and immunological liver injury. METHODS： The chronic and immunological hepatic liver injury was established in C57 BL/6J mice, the levels of ALT, AST, TP, ALB and HA in serum were determined, the contents of SOD, GSH and collagen 1 （C. I） in liver homogenate were detected, and the liver tissue sections were observed by electron microscope. RESULTS： Compared with DG, DG-PC could significantly improve the AST level, increase the content of ALB, increase the A/G, decrease the content of HA（P 〈 0.01）, improve the activities of SOD and GSH（ P 〈 0.01）. The therapeutic efficacy of DG-PC was better than that of DG. As to microstructure, DGPC could protect the hepatic cell and organelle, and relieve the fibrosis level. CONCLUSION： DG-PC has protective effects on chronic and immunological liver damage to C57 BL/6J mice, which maybe relate to the antioxidant effects.

关 键 词：甘草酸二铵磷脂复合物 慢性免疫性肝损伤 超微结构 

分 类 号：R965.2[医药卫生—药理学]