FRG4基因全长克隆及生物信息学分析  

The Full-Length cDNA Cloning and Bioinformatic Analysis of FRG4 Gene

作　　者：王仁[1] 姜志胜[1,2] 王佐[2] 赵战芝[2] 李国华[1] 杨向东[2] 

机构地区：[1]南华大学病理生理学教研室,湖南省衡阳市421001 [2]南华大学心血管病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2009年第6期426-430,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30570766;30470693);湖南省教育厅重点科研项目(05A043);湖南省教育厅基金(08C747);动脉硬化学湖南省高校科技创新团队资助项目(湘教通[2008]244);南华大学归国留学人员科研启动基金

摘　　要：为了对新的人突触相关蛋白FRG4进行全长克隆及生物信息学分析,从人胎肝文库PCR扩增FRG4基因全长cDNA序列,用生物信息学方法对FRG4基因进行基因组结构分析、多序列同源性比较、跨膜区段、亲疏水性分析、功能结构域预测等。结果表明cDNA文库基础上运用热启动PCR获得FRG4全长cDNA序列,生物信息学分析显示FRG4基因与人类的突触相关蛋白有99%同源性;定位在X染色体的短臂2区2带2亚带2次亚带;由9个外显子和8个内含子组成;无跨膜区段;有一结构域BSD;该基因编码蛋白可能为一水溶性蛋白。To analyze the basic character of a novel Homosapiens synapse associated protein gene （ FRG4 ） with bioinformatics. FRG4 full-length sequence was obtained by hot-start PCR from human fetal liver library and its genomie constitution, the multi-sequences homology, the transmembrane domains, hydrophilicity and hydrophobicity, as well as its functional and structural domains were analyzed and predicted by bioinformatics. We have successfully attained the fulllength cDNA sequence of FRG4, bioinformatic analysis shows FRG4 gene has 99% homology with Homosapiens synapse associated protein 1 （ SYAP1 ）, consists of 9 exons and 8 introns and located in Xp22.2. None transmembrane domains bat a BSD domain was discovered, and its encoding protein is probably a water-solubility protein.

关 键 词：生物信息学分析 动脉粥样硬化 突触相关蛋白 跨膜区 功能结构域 

分 类 号：Q81[生物学—生物工程]