青海湖鸟岛斑头雁种群对H5N1亚型禽流感病毒的免疫状况  

Immune Status to H5N1 Avian Influenza Virus of Bar-headed Geese on Bird Island in Qinghai Lake

作　　者：朱筱佳[1,2] 李来兴[1] 杨乐[1,2] 王贵华[3] 

机构地区：[1]中国科学院西北高原生物研究所高原生物适应和进化重点实验室,青海西宁810001 [2]中国科学院研究生院,北京100049 [3]中国科学院微生物研究所病原微生物学和免疫学重点实验室,北京100101

出　　处：《Zoological Research》2009年第4期406-410,共5页动物学研究（英文）

基　　金：国家科技支撑计划(2006BAD06A01);林业科技支撑计划(2008BADB0B0303)

摘　　要：斑头雁(Anserin dicus)是2005年青海湖H5N1型高致病性禽流感的主要被感染物种。为了解斑头雁目前对H5N1亚型禽流感病毒(AIV)免疫状况,2008年春季,在青海湖鸟岛采集该种群弃卵(68枚)和巢卵(125枚),以血凝抑制试验(HI)检测抗H5N1亚型禽流感病毒的卵黄母源抗体(IgY)。根据测试结果推断,在高致病性禽流感暴发3年后,青海湖鸟岛繁殖的斑头雁种群有26.5%～35.2%的繁殖对可能已经获得了对H5N1型禽流感病毒的免疫能力。另外,以斑头雁巢密度和抗体效价进行相关分析发现,斑头雁母源抗体水平与斑头雁巢密度正相关(r=0.736,P=0.000),表明高密度繁殖群内的母源抗体传递更具有适应性意义。The most infected species of H5N1 highly pathogenic avian influenza （HPAI） broke out in Qinghai Lake in 2005 was bar-headed goose （Anser indicus）. To investigate the immune status to H5N1-AIV of bar-headed geese in Qinghai Lake National Nature Reserve, 68 ejected eggs and 125 in-nest eggs of the breeding colony were collected in spring 2008, and haemagglutination inhibition （HI） was applied to analyze their yolk MAb （IgY） to H5N1-AIV. The results revealed, three years after the outbreak of HPAI in 2005, 26.5% to 35.2% of breeding pairs of the bar-headed geese breeding on Bird Island in Qinghai Lake may have acquired anti-H5N1 AIV immunocompetence. In addition, we analyzed the relationship of MAb titers of the nest-eggs and the nest density, and found the significant correlation between them （r=0.736, P=-0.000）. It is likely to be more adaptive that the MAb transmission pattern in the breeding colony with high breeding density.

关 键 词：斑头雁 H5N1型禽流感 卵黄 母源抗体 血凝抑制 青海湖 

分 类 号：S858.9[农业科学—临床兽医学]