三疣梭子蟹胚胎发育过程中肝胰腺的发生与卵黄物质利用的关系  

Relationship Between the Organogenesis of Hepatopancreas and the Yolk Utilization During Embryonic Development of Swimming Crab, Portunus trituberculatus

作　　者：吴旭干[1] 刘智俊[1] 姚桂桂[1] 成永旭[1] 杨筱珍[1] 王春琳[2] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室及上海市高校水产养殖学E-研究院,上海201306 [2]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《Zoological Research》2009年第4期449-456,共8页动物学研究（英文）

基　　金：国家自然科学基金(40806068);上海市晨光计划(2007CG64);国家"863"高科技研究发展计划项目(2006AA10A406);上海市教委水产养殖E-研究院建设项目(E03009)联合资助

摘　　要：通过对三疣梭子蟹胚胎进行连续采样和组织切片,系统研究了三疣梭子蟹胚胎发育过程中卵黄囊和肝胰腺的发生与卵黄物质利用的关系。结果表明:(1)三疣梭子蟹胚胎的卵黄岛和卵黄囊结构分别出现在原肠期和无节幼体期,胚胎从原肠期至卵内第一期溞状幼体期,始终存在卵黄岛结构,且卵黄岛中的卵黄物质不断被分解和利用.(2)卵内第二期溞状幼体后,卵黄囊分为两个区域,卵黄囊壁中出现肝胰腺细胞(柱状上皮细胞),此时肝胰腺前体已开始形成,卵黄岛开始融合.(3)卵内第三期溞状幼体阶段,卵黄囊发育成一双肝胰腺,由于肝胰腺中的卵黄物质互相融合,卵黄岛结构消失。此阶段胚胎对卵黄物质的利用加快,卵黄物质中存在许多空泡状结构;(4)胚胎发育进入孵化前期后,肝胰腺腔内的卵黄物质极少,而初孵溞状幼体肝胰腺腔内卵黄物质已完全消失,肝胰腺为一对囊状结构。这些结果表明在三疣梭子蟹胚胎发育从原肠期到孵化前的过程中,卵黄岛和肝胰腺细胞对于卵黄物质分解和利用起着十分重要的作用。By serial histological sections, embryos at different development stages were sampled and fixed to investigate the relationship between the organogenesis of yolk sac, hepatopancreas and the yolk utilization during the embryonic development of swimming crab, Portunus trituberculatus. The results showed that： （1） the formation of yolk island and yolk sac were in gastrula stage and egg nauplius stage, respectively, while the yolk islands could be found in the yolk sac from egg gastrula to egg Zoea I stage. （2） From egg Zoea Ⅱ stage, the yolk sac was divided into two parts because of further development of the midgut while the hepatopancreatic cells （columnar epithelium） were appearing in the inner of yolk sac membrane. This indicated the starting formation of hepatopancreas. （3） At egg Zoea Ⅲ stage, the yolk sac had been developed into a pair of hepatopancreas while the yolk from different yolk islands were combined, then all yolk islands disappeared from this stage. Due to yolk utilization, many vacuoles could be found in the hepatopancreatic lumen. （4） When the embryo developed into pre-hatching stage, the yolk in the lumen of hepatopancreas had been nearly depleted while for newly-hatched Zoea Ⅰ, the yolk had been completely utilized and hepatopancreas

关 键 词：三疣梭子蟹 胚胎发育 卵黄囊 肝胰腺 卵黄利用 组织学 

分 类 号：S917.4[农业科学—水产科学]