AFP 单克隆抗体的研制及其在免疫分析中的初步应用  

PRODUCTION OF AFP MONOCLONAL ANTIBODIES AND ITS APPLICATION IN IMMUNOASSAY

作　　者：刘一兵[1] 陈键[1] 韩世泉[1] 王衍真[1] 贺佑丰[1] 

机构地区：[1]中国原子能科学研究院同位素研究所

出　　处：《同位素》1998年第2期70-74,共5页Journal of Isotopes

摘　　要：获得了四株分泌ＡＦＰ（甲胎蛋白）单克隆抗体的杂交瘤细胞株并已稳定传代。其中Ａ４Ａ１１、Ａ１４Ｃ１１在ＡＦＰＩＲＭＡ中得到了很好的配对，经ＲＩＡ鉴定其滴度分别为１∶８００００和１∶１５００００，亲和常数分别为１．７×１０９Ｌ／ｍｏｌ和１．４×１０１０Ｌ／ｍｏｌ。Ａ１４Ｃ１１作为包被抗体应用于ＡＦＰＥＬＩＳＡ中也得到较好的结果。our cell strains of AFP monoclonal antibodies are obtained. Their titers are 1 ∶80 000—1∶150 000 and the affinity constants are in the range of (1.0-14.0)×10-9 L/mol. The crossreaction with Alb and IgG are less than 1.0×10-4% and 1.0×10-3% respectively. AFP IRMA is established with A4A11 and A14C11. The range of standard curve is 5-800 μg/L and sensitivity of assay is 1.93 μg/L. The recovery is 82.0%-94.8%.

关 键 词：甲胎蛋白 AFP单克隆抗体 肿瘤 免疫放射分析 

分 类 号：R817.4[医药卫生—影像医学与核医学] R730.3[医药卫生—放射医学]