鲍氏不动杆菌耐药及耐药与整合子相关性研究  

Correlation Between Integron and Drug-resistance in Clinical Isolates of Acinetobacter baumannii

作　　者：周东升[1] 唐吉斌[1] 潘晓龙[1] 徐元宏[2] 

机构地区：[1]铜陵市人民医院检验科,安徽铜陵244000 [2]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《中华医院感染学杂志》2009年第15期1925-1929,共5页Chinese Journal of Nosocomiology

基　　金：安徽省自然科学基金(050430804);安徽省铜陵市卫生局科研课题资助项目卫科研(2006.11)

摘　　要：目的调查医院鲍氏不动杆菌耐药谱并了解其整合子流行情况，检测鉴定整合子类型并分析其与耐药的相关性。方法收集医院2007年临床分离鲍氏不动杆菌85株，K—B法测定其药敏结果，PCR法扩增整合子的整合酶基因，并进一步鉴定整合子种类；分析鲍氏不动杆菌整合子携带与其耐药之间的关系；PCR法扩增整合子开放阅读框，观测其多态性并挑选个别产物测序。结果85株鲍氏不动杆菌除对亚胺培南、头孢哌酮／舒巴坦耐药率〈10．0％外，其他15种常用抗菌药物的耐药率均〉30．0％；67．1％的鲍氏不动杆菌中检出整合子；进一步鉴定结果显示均为Ⅰ类整合子，未检出Ⅱ类和Ⅲ类整合子；携带整合子鲍氏不动杆菌株对14种抗菌药物耐药率高于不携带整合子鲍氏不动杆菌株耐药率，开放阅读框可变区扩增产物为0．15～2．5kb条带，测序结果证实含有多药耐药基因编码。结论医院鲍氏不动杆菌耐药率高，以多药耐药菌株为主，携带Ⅰ类整合子率较高；携带整合子鲍氏不动杆菌对14种抗菌药物的耐药率明显增高，整合子与鲍氏不动杆菌的多药耐药具有密切相关性。OBJECTIVE To investigate the drug-resistant rates of clinical Acinetobacter baumannii strains isolated from our hospital and to understand the integrons distribution carried by A. baumannii. To identify the type of the integrons and analyze the correlation between integron and drug resistance in A. baumannii clinical isolates. METHODS Collected clinical isolates and the drug-sensitivity tests were completed by the method of KB. Amplified integrase were tested by PCR and identified the integron category. Correlation between integron in A. baumannii and drug resistance was analyzed. The open reading frame （ORF） of integron were amplified and observed the polymorphism of integron, Part of amplified products were sequenced. RESULTS Apart from imipenem and cefoperazone/sulbactam, the drug-resistant rate of 85 A. baumannii clinical isolates to else 15 kinds of antibacterials surpassed 30.0%. 67.1% A. baumannii clinical isolates carried the integrons, and confirmed all of them belonged to integron Ⅰ after identification, but integrons Ⅱ and Ⅲ were not found. The drug-resistant rates of A. baumannii with integron to 14 kinds of antibacterials were higher than those of A. baumannii without integron. The length of amplified products in variable region of ORF of integron varied from 0.15 to 2.5 kb. The sequencing results confirmed the integron carried multidrug-resistant gene code. CONCLUSIONS Drug-resistant rate of A. baumannii clinical isolates in our hospital is higher and the majority of them are multidrug-resistant strains. The drug-resistant rates of A. baurnannii with integron to 14 kinds of antibacterial are higher. The multidrug-resistant A. baumannii is associated with integrons.

关 键 词：鲍氏不动杆菌 整合子 耐药 多药耐药 

分 类 号：R378[医药卫生—病原生物学]