多药耐药铜绿假单胞菌β-内酰胺类耐药基因研究  

β-Lactamases associated Genes of Multi-resistant Pseudomonas aeruginosa in Guangzhou

作　　者：陈茶[1] 黄彬[2] 蓝锴[1] 庞雪云[1] 糜祖煌[3] 

机构地区：[1]广东省中医院检验科,广东广州510120 [2]中山大学附属第一医院检验医学部,广东广州510080 [3]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《中华医院感染学杂志》2009年第15期1930-1932,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(30770935)

摘　　要：目的进行多药耐药铜绿假单胞菌株中β-内酰胺酶耐药相关基因存在情况及耐药机制研究。方法用聚合酶链反应检测临床分离的22株多药耐药铜绿假单胞菌中的12种β-内酰胺酶基因(IMP、GIM、SPM、VIM、TEM、GES、VEB、PER、DHA、CARB、OXA-10、SHV)。结果22株菌中CARB基因全部阳性(100.0%),IMP基因阳性21株(阳性率95.5%),TEM基因阳性17株(阳性率77.3%),VEB基因仅3株阳性(13.6%),VIM基因1株阳性(4.5%),其余基因无阳性检出。结论铜绿假单胞菌中CARB及IMP基因携带率高,常常同时携带其他耐药基因,导致菌株出现多药耐药性,给临床诊治和医院感染监控带来极大的困难。OBJECTIVE To investigate β-lactamases genes in multi-resistant Pseudomonas aeruginosa in Guangzhou. METHODS We collected 22 P. aeruginosa strains from hospitalized patients, and detected eleven types of β-lactamases associated genes by PCR. RESULTS CARB, IMP, TEM, VEB and VIM showed positive amplifications with positive proportions were 100.0%, 95.5%, 77.3%, 13.6% and 4.5%, respectively. Other genes such as GIM, SPM, GES, PER, DHA, OXA-10 and SHV showed negative results. CONCLUSIONS The genes CARB and IMP have high positive rate. Different types of β-lactamases associated genes in P. aeruginosa aften lead to multi-resistance to antibiotics, which bring great difficulties to clinical therapy and hospital infection monitoring.

关 键 词：铜绿假单胞菌 Β-内酰胺酶 耐药基因 

分 类 号：R378.991[医药卫生—病原生物学]