22株多药耐药铜绿假单胞菌菌株亲缘性分析及耐药机制研究  

Antimicrobial-resistant Genes and Consanguinity in Multi-resistant Pseudomonas aeruginosa

作　　者：黄彬[1] 陈茶[2] 蓝锴[2] 庞雪云[2] 糜祖煌[3] 姜傥[1] 

机构地区：[1]中山大学附属第一医院检验医学部,广东广州510080 [2]广东省中医院检验科,广东广州510120 [3]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《中华医院感染学杂志》2009年第15期1933-1936,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(30770935)

摘　　要：目的研究22株多药耐药铜绿假单胞菌(PAE)菌株的亲缘性。方法采用聚合酶链反应(PCR)法检测22株多药耐药铜绿假单胞菌的β-内酰胺类耐药基因、氨基糖苷类修饰酶(AMEs)基因、喹诺酮类耐药基因、耐消毒剂基因(qacE△1-sul1)和整合酶基因,并以耐药基因为分子标志作聚类分析。结果22株菌中aac(6′)-Ⅰb、blaCARB、gyrA、oprD2、ant(2″)-Ⅰ和qacE△1-sul1基因的阳性率为100%,aac(3)-Ⅰ、aac(6′)-Ⅱ、blaGES、blaGIM、blaOXA-10群、blaPER、blaSPM、blaSHV和blaDHA基因均阴性,blaIMP-Ⅰ、blaTEM、blaVEB、aac(3)-Ⅱ、ant(3″)-Ⅰi、ntⅠ1和blaVIM基因的阳性率分别为95.5%、77.3%、13.6%、4.5%、4.5%、4.5%和4.5%;经样本聚类分析显示存在严重的克隆传播。结论22株铜绿假单胞菌β-内酰胺类、氨基糖苷类抗菌药物耐药与产β-内酰胺酶和氨基糖苷类修饰酶密切相关。医院感染铜绿假单胞菌可导致克隆传播医院感染,并引起暴发性流行。OBJECTIVE To investigate the antimicrobial-resistant genes and consanguinity in multi-resistant Pseudornonas aeruginosa. METHODS β-Lactamase genes, aminoglycoside modifiying enzymes （AMEs） genes, and quinolone resistance genes （qacE△1-sull） were detected in multi-resistant P. aeruginosa with polymerase chain reaction （PCR） and cluster analysis using drug resistance gene was conducted. RESULTS PCR results showed the positive ratios of genes aac（6＇）- Ⅰb, blaCARB, gyrA, oprD2, ant（2＂）-Ⅰ and qacE△1-sull were all 100%, blaIMP- Ⅰ , genes blaTEM, blaVEB, aac（3）- Ⅱ , ant（3＂）-Ⅰ , int Ⅰ 1 and blaVIM were 95.5%, 77.3%, 13.6%, 4.5%, 4.5%, 4.5% and 4.5%,respectively, while genes aac（3）- Ⅰ , aac（6＇）-Ⅱ , blaGES, blaGIM, blaOXA-10 group, blaPER, blaSPM, blaSHV and blaDHA were all negative. There was a clone transmitted phenomenon. CONCLUSIONS Multi-resistant P. aeruginosa can induce clone transmitted hospital infection and it has fulminant prevalence.

关 键 词：铜绿假单胞菌 多药耐药 耐药基因 聚类分析 

分 类 号：R378.991[医药卫生—病原生物学]