阴中求阳法治疗去卵巢骨质疏松大鼠骨组织作用的对比研究  

Effect of the prescription for reinforcing Yang from Yin on bone tissue of ovariectomy induced osteoporosis rats: A comparative study

作　　者：武密山[1] 赵素芝[2] 武中建 白霞[1] 郝秀红[2] 

机构地区：[1]河北医科大学中医学院,河北省石家庄市050091 [2]石家庄市桥东区医院,河北省石家庄市050041 [3]石家庄市桥东区妇幼保健站,河北省石家庄市050000

出　　处：《中国组织工程研究与临床康复》2009年第33期6478-6482,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30472200);河北省引进留学人员经费资助项目(200828)~~

摘　　要：背景:祖国医学中"肾主骨"的理论符合现代医学骨代谢调节机制,依此"阴中求阳法"和"阳中求阴法"理论研制的补肾复方在治疗骨质疏松症虽取得较好效果,但是滋补肾阴药与温补肾阳药配伍比例需要进行骨形成机制的深入探讨。目的:对比观察依照阴中求阳法组成的补肾方药中滋补肾阴药与温补肾阳药不同配伍比例对卵巢切除所致骨质疏松大鼠的治疗作用,以探讨它们之间的疗效是否存在差异。设计、时间及地点:随机对照动物实验,于2008-01/2009-01在河北医科大学中西医结合基础实验室完成。材料:清洁级3个月龄健康雌性SD大鼠(未曾交配)90只,只随机分成9组:正常对照组、假手术组、卵巢切除组、91组、82组、73组、64组、55组、补肾复方组,每组10只。补肾方药由地黄、淫羊藿、山药、丹参、骨碎补、独活等药物组成。方法:正常组和假手术组不造模,其余各组均切除卵巢造成骨质疏松症模型。91组、82组、73组、64组、55组分别按补肾阴药地黄∶补肾阳药淫羊藿为9∶1,8∶2,7∶3,6∶4及5∶5的比例配伍;生药含量均为960g/L,每天灌服7μL/g。补肾复方组按照地黄∶淫羊藿∶淮山药∶丹参∶骨碎补:独活等于8∶4∶4∶3∶3∶3的配伍比例制成的口服剂,生药含量约为1010g/L,每天灌服7μL/g,以上给药,1次/d,连续6d,休息1d后,再连续灌服6d,如此至3个月止;假手术组和正常对照组大鼠灌服等容积的蒸馏水。于卵巢切除1周后给药。主要观察指标:3个月后处死大鼠。普通光镜下观察骨小梁体积百分比、骨小梁吸收表面百分比及骨小梁形成表面百分比。荧光镜下观察活性生成表面百分比、骨小梁矿化率及骨小梁骨生成率。结果:90只大鼠均进入结果分析。切除卵巢3个月后,大鼠胫骨骨小梁体积百分比显著降低,骨小梁吸收表面百分比以及骨小梁形成表面百分比、活性生成表面百分比、�BACKGROUND： According to the theory of the kidney is in charge of the bones and the mechanism of bone metabolic regulation in modern medicine, the tonifying kidney prescriptions of reinforcing Yang from Yin and reinforcing Yin from Yang has effective action for osteoporosis, but further researches should focus on the action mechanism of the ratio of compatibility of the medicine for nourishing the Kidney-Yin and the medicine for warming and recuperating the Kidney-Yang at skeleton formation. OBJECTIVE： To compare the treatment effects of reinforcing Yang from Yin of the ratio of compatibility of the medicine for nourishing the Kidney-Yin and the medicine for warming and recuperating the Kidney-Yang on ovariectomy （OVX）-induced osteoporosis in rats. DESIGN, TIME AND SETTING： A randomized control animal experiment was carried out in the Basic Laboratory of Integrated Chinese Medicine and Western Medicine, Hebei Medical University between January 2008 and January 2009. MATERIALS： Ninety healthy female SD non-copulated rats of 3 months old and clean grade were randomly divided into nine groups： normal control group, sham operation group, OVX group, OVX plus 91 group, OVX plus 82 group, OVX plus 73 group, OVX plus 64 group, OVX plus 55 group and prescription of tonifying kidney group, with 10 rats in each group. The prescription for tonifying kidney comprised Rehmannia glutinosa, Epimedium grandiflorum, Dioscorea opposite, salviae miltiorrhizae, Drynaria fortunei and Angelica pubescens Maxim. METHODS： The osteoporosis model was induced in rats by the OVX with the exception of normal control group and sham operation group. The ratio of compatibility was 9：1, 8：2, 7：3, 6：4, 5：5 of Rehmannia glutinosa in prescription for nourishing the Kidney-Yin to Epimedium grandiflorum in prescription of warming and recuperating the Kidney-Yang, respectively. The crude drug content was 960 g/L, 7 μL/g gavage per day. The prescription of tonifying kidney consisted of Rehmannia glutinosa, Epimedium grandifloru

关 键 词：滋补肾阴方药 温补肾阳方药 卵巢切除术 骨质疏松 阴中求阳法 阴损及阳 

分 类 号：R681[医药卫生—骨科学]