联合应用多种血清学方法检测高危人群梅毒  

Syphilis Detection in High-risk Groups with Combined Serological Method

作　　者：韦善求 谢智光[2] 陆劲 

机构地区：[1]南宁市第七人民医院检验科,广西南宁530012 [2]广西瑞康医院检验科,广西南宁530011

出　　处：《中华医院感染学杂志》2009年第15期2061-2063,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨适合高危人群的梅毒血清学检测方法。方法对性病和妇科门诊有不洁性接触史、疑诊梅毒的高危患者采用梅毒螺旋体酶联免疫吸附试验(TP-ELISA)与甲苯胺红不加热血清试验(TRUST)法同步筛查,并对所有阳性结果进一步作苍白密螺旋体颗粒凝集试验(TPPA)确认。结果TP-ELISA与TRUST单一筛查法的检出率分别为18.3%、13.7%,两种方法同步筛查的检出率为20.8%;108份阳性结果经TPPA确认排除12.0%的假阳性。结论TP-ELISA与TRUST法同步筛查可以降低漏检率,用TPPA对筛查阳性的病例进一步确认可排除假阳性;3种方法联合应用能确保高危人群梅毒检测的灵敏度、准确性,且成本不高,可作为常规检测方法。OBJECTIVE To investigate syphilis serology detection methods suitable for high risk group. METHODS Treponerna pallidum （TP-ELISA） and toluidine red un-hoated serum test （TRUST） combined screening methods were taken for sexually transmitted disease （STD） patients and suspicious cases with dirty sexual intercourse, and all the positive results were further confirmed by TPPA method. RESULTS The detection rate of TP-ELISA or TRUST method alons was 18.3% or 13.7%, respectively. The detection rate of the both methods was 20.8%. A percent of 12.0% in 108 cases that were detected positively by the combined screening methods were proved to be false positive by TPPA. CONCLUSIONS The combined screening methods of TP-ELISA and TRUST can reduce the escaping rate, while TPPA can exclude the false positive cases in screening period. Sensitivity and accuracy of syphilis detection can be ensured by taking TP-ELISA and TRUST combined screening with TPPA.confirmation methods, which are without high cost and suitable for high risk group.

关 键 词：梅毒 梅毒螺旋体酶联免疫吸附试验 甲苯胺红不加热血清试验 苍白密螺旋体颗粒凝集试验 

分 类 号：R446.11[医药卫生—诊断学]