贮藏温度对UV-C辐射葡萄果皮白藜芦醇及其糖苷含量的影响  

Effects of Storage Temperature on Resveratrol and Its Glycosides Content in Grape Berries Skin Following UV-C Irradiation

作　　者：郑先波[1,2,3] 李晓东[2] 吴本宏[2] 李绍华[4] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]中国科学院植物研究所,北京100093 [3]河南农业大学园艺学院,郑州450002 [4]中国科学院武汉植物园,武汉430074

出　　处：《园艺学报》2009年第8期1099-1104,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30800744);中国科学院知识创新工程方向性项目(KSCX2-YW-N-032)

摘　　要：用剂量3.6kJ.m-2的紫外线C(Ultraviolet C,UV-C)辐射处理‘京蜜’葡萄成熟果实,25℃条件下避光孵育24h后,控制温度为25℃和0℃贮藏,贮藏后0、0.5、1、2、3、7、10、14、24、34d取样,HPLC法分析果皮白藜芦醇(Resveratrol,Res)及其糖苷含量。结果表明:UV-C辐射后25℃条件下孵育24h能显著提高反式白藜芦醇(trans-Resveratrol,trans-Res)和顺式白藜芦醇苷(cis-Piceid,cis-PD)的含量,不能提高反式白藜芦醇苷(trans-Piceid,trans-PD)含量。贮藏温度对UV-C辐射葡萄果皮Res及其糖苷含量具有重要影响。无论是对照果实还是UV-C辐射处理果实,果皮中均未检测到顺式白藜芦醇(cis-Resveratrol,cis-Res)。25℃和0℃贮藏条件下对照果皮trans-Res、trans-PD、cis-PD含量低,变化幅度很小,而UV-C处理果皮中变化幅度很大,绝大部分时间显著高于对照。The mature fruits of post-harvest ‘Jingmi＇ grape were irradiated by the dosage of 3, 6 kJ·2 m ultraviolet C （UV-C） and then incubated 24 hours in dark at 25℃. The UV-C irradiated berries and control berries were stored at 0℃ and 25℃. The berries was sampled at 0, 0. 5, 1, 2, 3, 7, 10, 14, 24 and 34 d. Resveratrol and piceid content in grape berries was determined by HPLC. The results showed： Trans-resveratrol （trans-Res） and c/s-Piceid （c/s-PD） content in grape berries skin was significantly increased when treated by UV-C irradiation and then incubated 24 h in dark at 25 ℃, but trans-Piceid （trans-PD） content in grape berries skin was not significantly increased. Storage temperature played an important role on the level of Res and its glycosides by UV-C irradiation treatment, c/s-Resveratrol （c/s-Res） could not detected in skin of UV-C irradiated berries and control berries, trans-Res, trans-PD and c/s-PD content of control grape berries skin was low and stable under 25℃ and 0℃, while UV-C irradiated berries skin was higher than control at mostly sampling time and changed sharply.

关 键 词：葡萄 贮藏 温度 UV-C辐射 白藜芦醇 白藜芦醇苷 

分 类 号：S663.1[农业科学—果树学]