Bt cry1Ia8抗虫基因对结球甘蓝的转化及其表达  

Transformation and Expressing of Bt Gene cry1Ia8 in Cabbage

作　　者：崔磊[1] 杨丽梅[1] 刘楠[2] 郎志宏[3] 刘玉梅[1] 庄木[1] 张扬勇[1] 张友军[1] 黄大昉[2,3] 方智远[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]中国农业科学院生物技术研究所,北京100081

出　　处：《园艺学报》2009年第8期1161-1168,共8页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2008AA10Z155);北京市科委项目(Z07070501770704)

摘　　要：以结球甘蓝(Brassica oleracea L.var.capitata L.)的下胚轴和具柄子叶为外植体,通过根癌农杆菌(Agrobacterium tumefaciens)介导法将苏云金芽孢杆菌(Bacillus thuringiensis)杀虫晶体蛋白基因cry1Ia8导入结球甘蓝中,共获得38株卡那霉素抗性植株。PCR和Southern blot检测证实cry1Ia8基因已经成功导入并整合到甘蓝的基因组中;RT-PCR和Western blot检测表明,cry1Ia8基因在转录和翻译水平有一定的表达;转化植株叶片离体饲喂敏感小菜蛾(Plutella xylostella)和对Cry1Ac蛋白表现抗性的小菜蛾的试验表明,该基因不仅对敏感小菜蛾有很强的抗性,对抗性小菜蛾也具有较强的抗性。In order to obtain transgenic cabbage lines with insect-resistance, cry1Ia8 gene was transferred to cabbage （Brassica oleracea L. vat. capitata L. ） with the transformation of hypocotyl segments and cotyledon with petiole via Agrobacterium tumefaciens. Thirty-eight transformants with kanamycin-resistance were obtained. PCR and Southern blot analysis showed that cry1Ia8 gene was integrated into the genome of cabbage. Moreover, RT-PCR and Western blot detection demonstrated that cry1Ia8 gene was expressed in RNA and protein levels. The results of bioassay with the susceptible and resistant diamondback moth （DBM, Plutella xylostella） displayed that most of the transgeuic plants were resistant to the larvae of susceptible DBM and also to the insect strain which is resistant to Cryl Ac toxin.

关 键 词：结球甘蓝 小菜蛾 BT基因 cry1Ia8 转基因 抗虫性 

分 类 号：S635.1[农业科学—蔬菜学] S436.341.24[农业科学—园艺学]