蜡梅天然群体遗传多样性的SRAP标记分析  

Genetic Diversity in Natural Populations of Chimonanthus praecox(L.) Link Revealed by SRAP Markers

作　　者：左丹丹[1] 赵海涛[1] 刘春[1] 穆鼎[1] 王晓武[1] 明军[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2009年第8期1197-1202,共6页Acta Horticulturae Sinica

基　　金：国家社会公益研究专项项目(2005DIB022);湖北省自然科学基金项目(2004ABA142);林木;花卉遗传育种教育部重点实验室开放基金项目(200605-12)

摘　　要：在蜡梅[Chimonanthus praecox(L.)Link]分布区内选取5个天然群体,采用筛选出的7对SRAP(sequence-related amplified polymorphism)引物进行遗传多样性分析。结果显示:多态性条带百分率为23.4%,多态位点比率(PPL)为83.41%,Neis基因多样性指数(h)为0.21～0.25(均值为0.22),Shannons信息指数(I)为0.34,群体分化系数(Gst)为0.17,群体间总的基因流的估算值Nm为2.40。AMOVA分析显示群体内方差分量的贡献率占79.89%,而群体间方差分量的贡献率占20.11%,区域间占32.39%,区域内为67.61%。Neis(72)群体遗传距离的UPGMA聚类分析显示群体间的遗传距离与群体的地理距离关系一致,Mantel分析相关系数为0.89。基于试验分析结果,蜡梅种质资源保护和利用策略为在优先保护现有群体的基础上,加强筛选和保存群体内的变异样本。The genetic diversity and genetic structure of natural populations of Chimonanthus praecox （L.） Link, were analyzed using Sequence-related Amplified Polymorphism （SRAP） markers. One hundred and fifty accessions from 5 natural populations were used in this study. Seven SRAP primer combinations revealed 170 legible bands of which 41 were polymorphic markers. The average proportion of polymorphic loci PPL was 83.41%. Gene diversity （h） was from 0.21 to 0. 25 （average 0. 22）, Shannon＇s Information index （I） was 0.34, coefficient of genetic differentiation （Gst） was 0. 17 and the mean of gene flow estimated （Nm） was 2.40. The results of AMOVA indicated that the variance components within populations and among populations contributing to total genetic variance were 79.89% and 20. 11% , among regions was 32. 39% and within regions were 67.61%. There was obvious genetic differentiation among populations and among regions. UPGMA cluster analysis based on Nei＇s genetic distance indicated that the genetic distances were closely related to corresponding geographic distances among populations, 2-way Mantel test Matrix correlation r was 0. 89. Conservation and utilization strategies for the species should be therefore emphasized on conserving as many as possible the existing populations, and selecting genetic diversity within populations considering the current conditions of severe population fragmentation and losses.

关 键 词：蜡梅 天然群体 遗传多样性 SRAP标记 

分 类 号：S685.17[农业科学—观赏园艺]