维生素E对体外培养人皮肤成纤维细胞透明质酸合成酶-2基因表达的影响  

Effects of vitamin E on the expression of hyaluronic acid synthetase-2 gene in human dermal fibroblasts in vitro

作　　者：范丽云[1] 刘全忠[1] 

机构地区：[1]天津医科大学总医院皮肤科,天津300052

出　　处：《中华医学美学美容杂志》2009年第4期217-219,共3页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：天津市自然科学基金资助项目（编号：003610211）

摘　　要：目的通过研究维生素E对体外培养的人皮肤成纤维细胞透明质酸合成酶-2（hyaluronic acid synthetase-2，HAS-2）mRNA转录水平的影响，探讨维生素E减缓皮肤老化的机制。方法采用酶消化法进行体外人皮肤成纤维细胞原代培养，然后将不同浓度的维生素E（0、1×10^-1、1×10^-9mol／L）加入体外培养的人皮肤成纤维细胞中，待药物作用24h后，提取总RNA，通过逆转录反应获得cDNA，并进行体外扩增。对扩增产物进行琼脂糖凝胶电泳，根据条带的亮度来判断成纤维细胞中HAS-2mRNA的表达水平。结果对体外培养的成纤维细胞进行维生素E干预，经B肌动蛋白内参校正后，反转录一聚合酶链式反应（RT—PCR）示低剂量组与对照组相比HAS-2mRNA表达水平的差异有统计学意义（P〈0．05），高剂量组与对照组相比HAS-2mRNA表达水平的差异有统计学意义（P〈0．05），低剂量组与高剂量组相比HAS-2mRNA表达水平的差异没有统计学意义（P〉0．05）。结论维生素E可以上调HAS-2基因的转录水平，可能增加皮肤成纤维细胞透明质酸的合成，增加皮肤的水分含量，使皮肤湿润、弹力增加、皱纹变浅，逆转或延缓皮肤老化。Objective To explore the mechanism of vitamin E on delaying skin aging by observing the expression of hyaluronic acid synthetase 2 （HAS-2） in human dermal fibroblasts in vitro. Methods Human skin fibroblasts were cultured in vitro, and these fibroblast ceils were then divided into 3 groups： different concentration of vitamin E （0,0、1×10^-1,1×10^-9mol/L） was added in the medium in the different group. 24 hours later, the fibroblasts were collected, RNAs extracted, and then amplified by RT-PCR. The PCR product was determined by agarose gel electrophoresis, to analyze the level of HAS 2 mRNA expression. Results RT-PCR showed the lever of HAS-2 mRNA was higher in the low-dose group than the control group, with significant difference （P〈0.05） ; the lever of HAS-2 mRNA was higher in the high dose group than control group, with significant difference （P〈0.05）. There was no significant difference in the lever of HAS-2 mRNA between the low-dose group and the high-dose group. Conclusions Vitamin E can enhance the expression of hyaluronic acid synthetase-2 mRNA, may increase the synthesis of HAS in skin fibroblasts and increase water content in the skin, so that it might reverse or delay the skin aging.

关 键 词：维生素E 透明质酸合成酶-2 成纤维细胞 皮肤老化 

分 类 号：R9[医药卫生—药学]