桂枝汤对自发性糖尿病大鼠心肌胶原重构的影响  

Effect of Guizhi Decoction on cardiac muscular collagen reconstruction in spontaneous diabetes rats

作　　者：李晓[1] 姜萍[1] 徐云生[1] 张勇[1] 姜月华[1] 

机构地区：[1]山东中医药大学附属医院,济南250011

出　　处：《中华中医药杂志》2009年第8期1068-1071,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省优秀中青年科学家基金(博士基金)(No.2005BSB02023)

摘　　要：目的:进一步桂枝汤对自发性糖尿病(GK)大鼠心肌Ⅰ型胶原、Ⅲ型胶原、血管紧张素Ⅱ的影响,为糖尿病心肌病变防治提供新治法新思路。方法:GK大鼠40只,随机分为GK对照组、二甲双胍组、桂枝汤组、黄连解毒汤组,分别灌胃12周,测定各组血糖、酶免法测定Ⅰ型胶原、Ⅲ型胶原,计算胶原Ⅰ/Ⅲ比值,放免法测定血管紧张素Ⅱ。结果:黄连解毒汤组、二甲双胍组血糖下降;桂枝汤组与GK对照组、二甲双胍比较,胶原Ⅰ明显下降,胶原Ⅲ明显升高,胶原Ⅰ/Ⅲ比值明显下降;桂枝汤组、黄连解毒汤与GK对照组、二甲双胍比较,心肌匀浆AT-Ⅱ含量均有下降。结论:桂枝汤能降低GK大鼠心肌匀浆胶原Ⅰ、AT-Ⅱ含量、升高胶原Ⅲ含量,降低胶原Ⅰ/Ⅲ比值,可明显防治GK大鼠心肌胶原重构和心肌纤维化。黄连解毒汤可降低AT-Ⅱ含量、对胶原Ⅰ、胶原Ⅰ/Ⅲ比值有降低趋势,对心肌胶原重构有一定改善作用。调和营卫是防治糖尿病心肌病的新治法、新思路。Objective： This study was to study effect of keeping ying and weiqi in balance on cardiac muscular collagen Ⅰ, collagen Ⅲ, angiotensin Ⅱ in spontaneous diabetes rats, and provide new thoughts for prevention and treatment for diabetic cardiomyopathy. Methods： 40 spontaneous diabetes rats were divided into four groups at random： spontaneous diabetes control group, diformin group, Guizhi Decoction group and Huanglian Jiedu Decoction group. Except control group, each group was given intragastric administration for 12weeks. Blood glucose, collagen I, collagen III, collagen I/collagen Ⅲ, angiotensin Ⅱwere determined. Euzymelinked immunosorbent assay and radio-immuniy method were used. Results： Blood glucose in Huanglian Jiedu Decoction group and diformin group decreased; Compared respectively with diformin group and control group, collagen I decreased obviously and collagen Ⅲ increased significantly in Guizhi Decoction group, and the collagen Ⅰ/collagen m decreased. Compared with diformin group and control group, cardiac muscular homogenate AT- Ⅱ contents decreased in both of Guizhi Decoction group and Huanglian Jiedu Decoction group. Conclusion： Guizhi Decoction can decrease the content of collagen I, AT- Ⅰ/ and collagen Ⅰ/collagen Ⅱ in cardiac muscular, and increase content of collagen Ⅱ, then prevent and treat cardiac muscular collagen reconstruction and myocardial fibrosis. Huanglian Jiedu Decoction can decrease the content of AT- Ⅱ, and decrease collagen Ⅰ/ collagen III, collagen I slightly, and can improve cardiac muscular collagen reconstruction. Keeping ying and weiqi in balance is a new thought for prevention and treatment for diabetic cardiomyopathy.

关 键 词：Ⅰ型胶原 Ⅲ型胶原 血管紧张素Ⅱ 胶原重构 自发性糖尿病大鼠 黄连解毒汤 桂枝汤 

分 类 号：R285.5[医药卫生—中药学]