土壤微生物多样性研究的DGGE/TGGE技术进展  

ADVANCES OF DGGE/TGGE TECHNIQUE FOR SOIL MICROBIAL DIVERSITY STUDY

作　　者：张洪霞[1] 谭周进[2] 张祺玲[1] 盛荣[1,3] 肖和艾[3] 

机构地区：[1]湖南农业大学生物安全科技学院,湖南长沙410128 [2]湖南中医药大学基础医学院,湖南长沙410208 [3]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《核农学报》2009年第4期721-727,共7页Journal of Nuclear Agricultural Sciences

基　　金：中国科学院知识创新工程项目(KZCX2-YW-408);国家自然科学基金(40771116);国家支撑计划项目课题(2008BADA7B09)

摘　　要：分子生物学技术比传统的培养方法可得到土壤微生物种群多样性更全面的信息。变性梯度凝胶电泳(Denaturing gradient gel electrophoresis,DGGE)和温度梯度凝胶电泳(Temperature gradient gel electrophoresis,TGGE)可分离PCR扩增的DNA片段,已成为研究土壤微生物群落多样性的重要手段。本文综述了DGGE/TGGE技术在土壤微生物多样性研究中的应用进展,分析了该方法的主要影响因素及其优点和存在的问题。Molecular biological technology can be used to research and characterize soil microbes which currently can not be cultured by traditional microbial research methods,and to get more and more complete information about soil microbial community,to reveal soil microbial population structure and genetic diversity more accurately.Denaturing gradient gel electrophoresis（DGGE） and temperature gradient gel electrophoresis（TGGE） techniques had been proven to be powerful tools for analyzing microbial diversity,which can directly separating the PCR amplification clips.In this paper,the influence factors and application aspects,as well as the potentials and the limitations of DGGE/TGGE techniques for microbial diversity analysis were reviewed.

关 键 词：土壤微生物 多样性 DGGE TGGE 

分 类 号：S154.3[农业科学—土壤学] Q503[农业科学—农业基础科学]