羊栖菜多糖对离体小鼠NK细胞活性和巨噬细胞功能的影响  

Effects of Sargassum fusiforme polysaccharides on mouse NK cell activity and the function of mouse peritoneal macrophage in vitro

作　　者：陈慧玲[1] 况炜[1] 章皓[1] 鲍建芳[2] 

机构地区：[1]宁波天一职业技术学院,浙江宁波315104 [2]浙江大学医学院,浙江杭州310058

出　　处：《现代实用医学》2009年第7期691-692,695,共3页Modern Practical Medicine

基　　金：宁波市医学科技项目;编号2004070

摘　　要：目的观察羊栖菜多糖对离体小鼠NK细胞活性和巨噬细胞功能的影响。方法10、30、100mg/L浓度的羊栖菜多糖分别作用小鼠脾细胞6、12和18h,采用四甲基偶氮唑盐(MTT)比色法测定NK细胞对YAC-1细胞的杀伤活性;10、30、100mg/L浓度的羊栖菜多糖分别作用小鼠腹腔巨噬细胞16、24和48h,测定其所分泌的一氧化氮(NO)量。结果10、30mg/L浓度组的羊栖菜多糖作用6、12h对离体小鼠NK细胞活性有明显的促进作用,以30mg/L浓度羊栖菜多糖作用组效果最为显著(<0.01);10、30mg/L浓度组的羊栖菜多糖促进小鼠腹腔巨噬细胞分泌NO作用更为明显,以10mg/L浓度组作用48h效果最为显著(<0.01)。结论羊栖菜多糖能明显增强离体小鼠NK细胞活性,促进巨噬细胞释放NO。Objective To observe effects of Sargassum fusiforme polysaccharides（SFPS）on mouse NK cell activity and the function of mouse peritoneal macrophages in vitro.Methods SFPS had treated the spleen cells of mice for 6、12 and 18 hours in different concentration（10 mg/L、30 mg/L、100 mg/L）,NK cell activity were measured by MTT method.Mouse peritoneal macrophages had been treated by SFPS for 16、24 and 48 hours in different concentration（10 mg/L、30 mg/L、100 mg/L）,the levels of nitric oxide were assessed.Results SFPS（10 mg/L、30 mg/L）significantly enhanced NK cell activity after treating the spleen cells of mice for 6 and 12 hours,and the effect of 30 mg/L concentration group was the most obviou（P 〈 0.01）.Levels of nitric oxide of mouse peritoneal macrophages were increased more obviously after macrophages were treated with 10 mg/L and 3 0mg/L SFPS,and the effect was the most significant after treated with SFPS for 48 hour（P 〈 0.01）.Conclusion SFPS could obviously enhance mouse NK cell activity and promote the release of nitric oxide in mouse peritoneal macrophages in vitro.

关 键 词：小鼠 羊栖菜多糖 巨噬细胞 NK细胞 

分 类 号：R282.77[医药卫生—中药学]