再生柞蚕丝素蛋白对小鼠间充质干细胞体外生长的支持作用  

Effect of Regenerated Antheraea Penyi Silk Fibroin on the in vitro Proliferation of the Mouse Mesenchymal Stem Cell

作　　者：谢炜[1] 王泳[1] 古彦铮[1] 潘建忠[1] 徐颖[1] 徐云云[1] 李明忠[2] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所,江苏苏州215007 [2]苏州大学材料工程学院,江苏苏州215021

出　　处：《苏州大学学报（医学版）》2009年第3期403-405,410,共4页Suzhou University Journal of Medical Science

基　　金：国家重点基础研究发展计划(973计划)项目(2005CB623906)

摘　　要：目的探讨再生柞蚕丝素蛋白对小鼠间充质干细胞体外生长和分化的支持作用。方法采用再生家蚕丝素蛋白、再生柞蚕丝素蛋白、I型胶原、普通细胞培养板为研究对象,观察小鼠间充质干细胞(C3H10T1/2)在这4种生物材料上的黏附、生长及表面抗原的变化。利用光学显微镜观察细胞生长形态,MTT法检测细胞的增殖,流式细胞仪测定细胞表型。结果再生柞蚕丝素蛋白对细胞生长形态、细胞表面抗原表达均无影响。培养第6天C3H10T1/2细胞在再生柞蚕丝素膜上增殖明显,与其他3种生物材料的差异有统计学意义(P<0.05)。结论再生柞蚕丝素蛋白在体外支持C3H10T1/2细胞的黏附、生长,具有很好的组织相容性。：Objective To explore the growth of the mouse mesenchymal stem cell line C3H10T1/2 on the regenerated antheraea penyi silk fibroin membrane. Methods Four biomaterials were used in the study, such as the regenerated silk fibroin of domestic silkworm （B mori SF）, the regenerated silk fibroin of antheraea penyi （A penyi SF）, collagen and the cell culture plastic. The attachment, proliferation and the phenotypes of the C3H10T1/2 cell line cultured on the four biomaterials were investigated in detail. The morphology of the cells was observed by the inverted microscope. The viability of the C3H10T1/2 cells was detected by MTT assay. The phenotypes of the C3H10T1/2 cell were assayed by flow cytometry. Results The viability of C3H10T1/2 on the antheraea penyi material was significantly higher than that on the other biomaterials on the sixth day （P〈0.05）. Culture on the antheraea penyi did not change the expression of surface antigens of C3H10T1/2 which was analyzed by flow cytometry. In addition, culture on the antheraea penyi did not change the morphology of the C3H10T1/2 cells. Conclusion The regenerated silk fibroin of antheraea penyi can support C3H10T1/2 cell attachment and growth in vitro, which demonstrate its good biocompatibilities.

关 键 词：小鼠间充质干细胞 再生柞蚕丝素蛋白 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]