土霉素、甲烯土霉素和强力霉素的化学发光分析法  

Study on Chemiluminescence Method for Oxytetracycline,Methacycline and Deoxycycline

作　　者：黎朝[1] 王尊本[1] 

机构地区：[1]厦门大学化学系材料和生命过程分析科学国家教委开放研究实验室

出　　处：《厦门大学学报（自然科学版）》1998年第4期547-552,共6页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金

摘　　要：在碱性介质中，铜氨络离子会催化过氧化氢与土霉素、甲烯土霉素或强力霉素的化学发光反应，表面活性剂溴化十六烷基三甲基铵（ＣＴＭＡＢ）对该反应具有增敏作用．发光强度与土霉素、甲烯土霉素或强力霉素的含量分别在８．０×１０－８～８．０×１０－６ｍｏｌ／Ｌ、５．０×１０－８～５．０×１０－６ｍｏｌ／Ｌ和１．０×１０－７～１．０×１０－５ｍｏｌ／Ｌ的范围内分两个量程呈线性关系．７次平行测定的相对标准偏差分别为１．８％～４．１％、２．４％～３．５％和２．５％～４．０％．标准加入的回收率为９９．６％～１０１．４％、９９．１％～１０２．３％和９９．４％～１０１．４％．应用于尿液中这３种四环素类药物的回收测定。The chemiluminescence reactions of oxytetracycline ( OTC ), methacycline ( MC) or deoxycycline(DC) with hydrogen peroxide were catalysed by the [Cu(NH3)4]2+ ion in ammonia medium.The CL intensities were enhanced by CTMAB. The CL intensities were linear with their contents in 8.0×10-8～8.0×10-6, 5.0×10-8 ～5.0×10-6 and 1.0×10-7～5.0×10-5 mol/L for OTC,MC and DC respectively. The relative standard deviations for 7 determinations were from 1.8～4.1,2.4～3.5 and 2.5～4.0 % for OTC,MC and DC respectively. The recoveries were from 99.6～101.4,99.1～102.3 and 99.4～101.4 % for OTC,MC and DC respectively . This method can be used to the determinations of OTC,MC and DC in urine with satisfactory results.

关 键 词：化学发光 土霉素 甲烯土霉素 强力霉素 药物分析 

分 类 号：R978[医药卫生—药品] R914.1[医药卫生—药学]