血液中异山梨醇[1.4;3.6]二醚-5-单硝酸酯的测定  

Determination of Isosorbide 5 mononitrate in Human Plasma

作　　者：王永华[1] 黄建华 黄爱今[2] 孙亦梁[2] 崔华东 黄一玲 

机构地区：[1]北京大学城市与环境学系 [2]北京大学化学与分子工程学院 [3]中国医学科学院协和医科大学北京阜外心血管病医院临床药理研究室

出　　处：《北京大学学报（自然科学版）》1998年第4期467-470,共4页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：建立了一种改进的简单、灵敏、准确地测定血液中异山梨醇［１，４；３，６］二醚－５－单硝酸酯的毛细柱气相色谱方法。血浆样品加入饱和Ｎａ２ＣＯ３溶液，以乙醚作萃取剂，甲醇重溶解，２，４－二硝基甲苯作内标物，最低检出限可达５μｇ／Ｌ。标准曲线相关系数大于０．９９９。对高中低３种浓度血浆样品的日内和日间相对误差和相对标准偏差均小于８％。回收率９２－１０４％。An improved,sensitive and precise capillary gas chromatographic method with electron capture detector has been developed for the determination of isosorbide 5 mononitrate in human plasma.2,4 Dinatrotoluene is used as the internal standard.The plasma is extracted with ether in sodium carbonate solution.The residue was reconstructed with methanol.The linear correlation coefficient between concentration and height ratio is better than 0.999.The recovery was found to range from 92% to 104% for three concentration levels of samples with a limit of determination 5 μg/L and with the relative standard deviation of 8% within day.

关 键 词：气相色谱 血液 IS-5-MN 抗心绞痛药物 

分 类 号：R972.3[医药卫生—药品]