TTC-脱氢酶活性测定中标准曲线的影响因素研究  

Study on influencing factors for standard curve of TTC-Dehydrogenase activity measurement

作　　者：赵连梅[1] 池勇志[1] 张春青[1] 

机构地区：[1]天津城市建设学院环境与市政工程系,天津300384

出　　处：《实验室科学》2009年第4期72-74,共3页Laboratory Science

基　　金：天津市自然科学基金(08JCYBJC13200)

摘　　要：此文采用722S型可见分光光度计,以连二亚硫酸钠为还原剂,对传统TTC-脱氢酶活性测定法中影响标准曲线制作的因素进行了研究,确定了最佳测定条件为:最大吸收峰在475nm处;连二亚硫酸钠的最佳投量为5.0mg/5mL;TTC溶液的保存及整个实验过程应避光。最佳反应时间为30min。In this paper the factors which influence the standard curve of determination are investigated by using 722S type visible pectrophotometer and sodium hydrosulfite as reductant in the measure method conventional TIC - Dehydrogenase activity. The optimum conditions are as follows： the maximum absorption peak is at the point of 475 nm; the best dosage of sodium dithionite is 5. 0 mg/5mL ; preservation of TTC solution and the whole test process should be in darkness; the optimum reaction time is 30 rain.

关 键 词：TTC 脱氢酶活性 标准曲线 

分 类 号：X705[环境科学与工程—环境工程]